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Human PYY (Peptide YY) Sandwich ELISA (STJE0002816)

Human PYY (Peptide YY) Sandwich ELISA (STJE0002816)
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  • 产品名称:Human PYY (Peptide YY) Sandwich ELISA (STJE0002816)
  • 产品型号:STJE0002816
  • 产品展商:ST john's laboratory
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简单介绍
Human PYY (Peptide YY) Sandwich ELISA (STJE0002816)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 18.75pg/mL
Detection Limit: 31.25~2000pg/mL
Short Description: This PYY Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: PYY
Gene ID: 5697
Uniprot ID: PYY_HUMAN
Specificity: This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function This gut peptide inhibits exocrine pancreatic secretion, has a vasoconstrictory action and inhibitis jejunal and colonic mobility.
Protein Name Peptide Yy
Pyy
Pyy-I
Peptide Tyrosine Tyrosine Cleaved Into - Peptide Yy(3-36
Pyy-Ii
Database Links Reactome: R-HSA-375276
Reactome: R-HSA-418594
Cellular Localisation Secreted
Alternative ELISA Names Peptide Yy ELISA kit
Pyy ELISA kit
Pyy-I ELISA kit
Peptide Tyrosine Tyrosine Cleaved Into - Peptide Yy(3-36 ELISA kit
Pyy-Ii ELISA kit
PYY ELISA kit
Specificity This kit recognizes Human PYY in samples. No significant cross-reactivity or interference between Human PYY and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-105 99
EDTA plasma (n=8) 85-100 91
Cell culture media (n=8) 89-103 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 105.33 303.30 971.46 107.16 318.05 955.53
Standard deviation 5.72 15.23 49.74 6.19 17.40 38.51
CV (%) 5.43 5.02 5.12 5.78 5.47 4.03
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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