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Human SELE (E-selectin) CLIA Kit (STJC0000440)

Human SELE (E-selectin) CLIA Kit (STJC0000440)
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  • 产品名称:Human SELE (E-selectin) CLIA Kit (STJC0000440)
  • 产品型号:STJC0000440
  • 产品展商:ST john's laboratory
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简单介绍
Human SELE (E-selectin) CLIA Kit (STJC0000440)
产品描述
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This human SELE kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: SELE
Gene ID: 6401
Uniprot ID: LYAM2_HUMAN
Specificity: This kit recognizes Human SELE in samples. No significant cross-reactivity or interference between Human SELE and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Cell-surface glycoprotein having a role in immunoadhesion. Mediates in the adhesion of blood neutrophils in cytokine-activated endothelium through interaction with SELPLG/PSGL1. May have a role in capillary morphogenesis.
Protein Name E-Selectin
Cd62 Antigen-Like Family Member E
Endothelial Leukocyte Adhesion Molecule 1
Elam-1
Leukocyte-Endothelial Cell Adhesion Molecule 2
Lecam2
Cd Antigen Cd62e
Database Links Reactome: R-HSA-202733
Cellular Localisation Cell Membrane
Single-Pass Type I Membrane Protein
Alternative CLIA Names E-Selectin CLIA kit
Cd62 Antigen-Like Family Member E CLIA kit
Endothelial Leukocyte Adhesion Molecule 1 CLIA kit
Elam-1 CLIA kit
Leukocyte-Endothelial Cell Adhesion Molecule 2 CLIA kit
Lecam2 CLIA kit
Cd Antigen Cd62e CLIA kit
SELE CLIA kit
ELAM1 CLIA kit
Specificity This kit recognizes Human SELE in samples. No significant cross-reactivity or interference between Human SELE and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 98-112 106
EDTA plasma(n=8) 90-102 95
Cell culture media(n=8) 99-115 107

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 49.02 122.60 405.49 45.89 131.36 437.19
Standard deviation 4.37 13.15 36.90 4.51 10.61 31.57
CV (%) 8.91 10.73 9.10 9.83 8.08 7.22
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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