Human SOD2 (Superoxide Dismutase 2, Mitochondrial) Sandwich ELISA (STJE0019792) STJE0019792 上海玉博生物科技有限公司

Human SOD2 (Superoxide Dismutase 2, Mitochondrial) Sandwich ELISA (STJE0019792)

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产品名称：Human SOD2 (Superoxide Dismutase 2, Mitochondrial) Sandwich ELISA (STJE0019792)
产品型号：STJE0019792
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human SOD2 (Superoxide Dismutase 2, Mitochondrial) Sandwich ELISA (STJE0019792)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.19ng/mL
Detection Limit:	0.31~20ng/mL
Short Description:	This SOD2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	SOD2
Gene ID:	6648
Uniprot ID:	SODM_HUMAN
Specificity:	This kit recognizes Human SOD2 in samples. No significant cross-reactivity or interference between Human SOD2 and analogues was observed.
Sample Type:	serum, plasma and other biological fluids

Post Translational Modifications	Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity. Acetylation at Lys-122 decreases enzymatic activity. Deacetylated by SIRT3 upon exposure to ionizing radiations or after long fasting. Polyubiquitinated.leading to proteasomal degradation. Deubiquitinated by USP36 which increases protein stability.
Function	Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Protein Name	Superoxide Dismutase Mn - Mitochondrial
Database Links	Reactome: R-HSA-2151201 Reactome: R-HSA-3299685 Reactome: R-HSA-8862803 Reactome: R-HSA-8950505 Reactome: R-HSA-9615017
Cellular Localisation	Mitochondrion Matrix
Alternative ELISA Names	Superoxide Dismutase Mn - Mitochondrial ELISA kit SOD2 ELISA kit
Specificity	This kit recognizes Human SOD2 in samples. No significant cross-reactivity or interference between Human SOD2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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