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Human SOD3 (Extracellular superoxide dismutase) Competitive ELISA (STJE0003664)

Human SOD3 (Extracellular superoxide dismutase) Competitive ELISA (STJE0003664)
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  • 产品名称:Human SOD3 (Extracellular superoxide dismutase) Competitive ELISA (STJE0003664)
  • 产品型号:STJE0003664
  • 产品展商:ST john's laboratory
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简单介绍
Human SOD3 (Extracellular superoxide dismutase) Competitive ELISA (STJE0003664)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 3.75ng/mL
Detection Limit: 6.25~400ng/mL
Short Description: This SOD3 Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 2.5h
Detection: Colormetric
Gene Symbol: SOD3
Gene ID: 6649
Uniprot ID: SODE_HUMAN
Specificity: This kit recognizes Human SOD3 in samples. No significant cross-reactivity or interference between Human SOD3 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Function Protect the extracellular space from toxic effect of reactive oxygen intermediates by converting superoxide radicals into hydrogen peroxide and oxygen.
Protein Name Extracellular Superoxide Dismutase Cu-Zn
Ec-Sod
Database Links Reactome: R-HSA-3299685
Cellular Localisation Secreted
Extracellular Space
Golgi Apparatus
Trans-Golgi Network
99% Of Ec-Sod Is Anchored To Heparan Sulfate Proteoglycans In The Tissue Interstitium
And 1% Is Located In The Vasculature In Equilibrium Between The Plasma And The Endothelium
Alternative ELISA Names Extracellular Superoxide Dismutase Cu-Zn ELISA kit
Ec-Sod ELISA kit
SOD3 ELISA kit
Specificity This kit recognizes SOD3 in samples. No significant cross-reactivity or interference between SOD3 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8℃
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-108 99
EDTA plasma(n=8) 87-100 93
Cell culture media(n=8) 91-104 96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (ng/mL) 20.30 63.40 182.70 22.10 66.30 166.50
Standard deviation 1.30 2.70 5.70 1.20 3.00 7.20
CV (%) 6.40 4.26 3.12 5.43 4.52 4.32
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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