Human SPARC (SPARC) CLIA Kit (STJC0000619) STJC0000619 上海玉博生物科技有限公司

Human SPARC (SPARC) CLIA Kit (STJC0000619)

简单介绍

Human SPARC (SPARC) CLIA Kit (STJC0000619)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	37.5pg/mL
Detection Limit:	62.5~4000pg/mL
Short Description:	This human SPARC kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	SPARC
Gene ID:	6678
Uniprot ID:	SPRC_HUMAN
Specificity:	This kit recognizes Human ON in samples. No significant cross-reactivity or interference between Human ON and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Appears to regulate cell growth through interactions with the extracellular matrix and cytokines. Binds calcium and copper, several types of collagen, albumin, thrombospondin, PDGF and cell membranes. There are two calcium binding sites.an acidic domain that binds 5 to 8 Ca(2+) with a low affinity and an EF-hand loop that binds a Ca(2+) ion with a high affinity.
Protein Name	Sparc Basement-Membrane Protein 40 Bm-40 Osteonectin On Secreted Protein Acidic And Rich In Cysteine
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-1251985 Reactome: R-HSA-3000178 Reactome: R-HSA-3000497
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix Basement Membrane In Or Around The Basement Membrane
Alternative CLIA Names	Sparc CLIA kit Basement-Membrane Protein 40 CLIA kit Bm-40 CLIA kit Osteonectin CLIA kit On CLIA kit Secreted Protein Acidic And Rich In Cysteine CLIA kit SPARC CLIA kit ON CLIA kit
Specificity	This kit recognizes Human ON in samples. No significant cross-reactivity or interference between Human ON and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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