Human SPX (Spexin) Sandwich ELISA (STJE0003973) STJE0003973 上海玉博生物科技有限公司

Human SPX (Spexin) Sandwich ELISA (STJE0003973)

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产品名称：Human SPX (Spexin) Sandwich ELISA (STJE0003973)
产品型号：STJE0003973
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human SPX (Spexin) Sandwich ELISA (STJE0003973)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	46.88pg/mL
Detection Limit:	78.13~5000pg/mL
Short Description:	This SPX Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	SPX
Gene ID:	80763
Uniprot ID:	SPXN_HUMAN
Specificity:	This kit recognizes Human SPX in samples. No significant cross-reactivity or interference between Human SPX and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Function	Plays a role as a central modulator of cardiovascular and renal function and nociception. Also plays a role in energy metabolism and storage. Inhibits adrenocortical cell proliferation with minor stimulation on corticosteroid release. Spexin-1: Acts as a ligand for galanin receptors GALR2 and GALR3. Intracerebroventricular administration of the peptide induces an increase in arterial blood pressure, a decrease in both heart rate and renal excretion and delayed natriuresis. Intraventricular administration of the peptide induces antinociceptive activity. Also induces contraction of muscarinic-like stomach smooth muscles. Intraperitoneal administration of the peptide induces a reduction in food consumption and body weight. Inhibits long chain fatty acid uptake into adipocytes. Spexin-2: Intracerebroventricular administration of the peptide induces a decrease in heart rate, but no change in arterial pressure, and an increase in urine flow rate. Intraventricular administration of the peptide induces antinociceptive activity.
Protein Name	Spexin Npq Neuropeptide Q Spexin Hormone Cleaved Into - Spexin-1 - Spexin-2 Npq 53-70
Cellular Localisation	Secreted Extracellular Space Cytoplasmic Vesicle Secretory Vesicle Secreted Via The Classical Er/Golgi-Dependent Pathway Into The Extracellular Medium Largely As A Full-Length Protein Without The Signal Peptide And Not As A Hydrolyzed And Amidated Peptide Localized Extracellularly Surrounding The Villous Trophoblastic Cells Detected In The Serum
Alternative ELISA Names	Spexin ELISA kit Npq ELISA kit Neuropeptide Q ELISA kit Spexin Hormone Cleaved Into - Spexin-1 - Spexin-2 ELISA kit Npq 53-70 ELISA kit SPX ELISA kit C12orf39 ELISA kit
Specificity	This kit recognizes Human SPX in samples. No significant cross-reactivity or interference between Human SPX and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	93-109	101
EDTA plasma (n=8)	94-107	99
Cell culture media (n=8)	91-106	97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	252.13	716.26	1809.17	272.69	679.36	1914.47
Standard deviation	13.87	40.61	92.63	15.24	29.96	79.83
CV (%)	5.50	5.67	5.12	5.59	4.41	4.17

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human SPX (Spexin) Sandwich ELISA (STJE0003973)