Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) Sandwich ELISA (STJE0003931) STJE0003931 上海玉博生物科技有限公司

Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) Sandwich ELISA (STJE0003931)

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产品名称：Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) Sandwich ELISA (STJE0003931)
产品型号：STJE0003931
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) Sandwich ELISA (STJE0003931)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	9.38pg/mL
Detection Limit:	15.63~1000pg/mL
Short Description:	This sRANKL Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	TNFSF11
Gene ID:	8600
Uniprot ID:	TNF11_HUMAN
Specificity:	This kit recognizes Human sRANKL in samples. No significant cross-reactivity or interference between Human sRANKL and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	The soluble form of isoform 1 derives from the membrane form by proteolytic processing. The cleavage may be catalyzed by ADAM17.
Function	Cytokine that binds to TNFRSF11B/OPG and to TNFRSF11A/RANK. Osteoclast differentiation and activation factor. Augments the ability of dendritic cells to stimulate naive T-cell proliferation. May be an important regulator of interactions between T-cells and dendritic cells and may play a role in the regulation of the T-cell-dependent immune response. May also play an important role in enhanced bone-resorption in humoral hypercalcemia of malignancy. Induces osteoclastogenesis by activating multiple signaling pathways in osteoclast precursor cells, chief among which is induction of long lasting oscillations in the intracellular concentration of Ca (2+) resulting in the activation of NFATC1, which translocates to the nucleus and induces osteoclast-specific gene transcription to allow differentiation of osteoclasts. During osteoclast differentiation, in a TMEM64 and ATP2A2-dependent manner induces activation of CREB1 and mitochondrial ROS generation necessary for proper osteoclast generation.
Protein Name	Tumor Necrosis Factor Ligand Superfamily Member 11 Osteoclast Differentiation Factor Odf Osteoprotegerin Ligand Opgl Receptor Activator Of Nuclear Factor Kappa-B Ligand Rankl Tnf-Related Activation-Induced Cytokine Trance Cd Antigen Cd254 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 11 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 11 - Soluble Form
Database Links	Reactome: R-HSA-5668541 Reactome: R-HSA-5669034 Reactome: R-HSA-5676594
Cellular Localisation	Isoform 1: Cell Membrane Single-Pass Type Ii Membrane Protein Isoform 3: Cell Membrane Isoform 2: Cytoplasm Tumor Necrosis Factor Ligand Superfamily Member 11 Soluble Form: Secreted
Alternative ELISA Names	Tumor Necrosis Factor Ligand Superfamily Member 11 ELISA kit Osteoclast Differentiation Factor ELISA kit Odf ELISA kit Osteoprotegerin Ligand ELISA kit Opgl ELISA kit Receptor Activator Of Nuclear Factor Kappa-B Ligand ELISA kit Rankl ELISA kit Tnf-Related Activation-Induced Cytokine ELISA kit Trance ELISA kit Cd Antigen Cd254 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 11 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 11 - Soluble Form ELISA kit TNFSF11 ELISA kit OPGL ELISA kit RANKL ELISA kit TRANCE ELISA kit
Specificity	This kit recognizes Human sRANKL in samples. No significant cross-reactivity or interference between Human sRANKL and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	92-104	99
EDTA plasma (n=8)	88-101	93
Cell culture media (n=8)	92-102	97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	52.28	122.42	372.33	55.30	121.01	370.30
Standard deviation	3.20	5.66	17.61	3.65	5.14	16.11
CV (%)	6.12	4.62	4.73	6.60	4.25	4.35

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human sRANKL (Soluble Receptor Activator of Nuclear factor-kB Ligand) Sandwich ELISA (STJE0003931)