Human STAT4 (Signal transducer and activator of transcription 4) CLIA Kit (STJC0000895) STJC0000895 上海玉博生物科技有限公司

Human STAT4 (Signal transducer and activator of transcription 4) CLIA Kit (STJC0000895)

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产品名称：Human STAT4 (Signal transducer and activator of transcription 4) CLIA Kit (STJC0000895)
产品型号：STJC0000895
产品展商：ST john's laboratory
产品文档：无相关文档

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Human STAT4 (Signal transducer and activator of transcription 4) CLIA Kit (STJC0000895)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	37.5pg/mL
Detection Limit:	62.5~4000pg/mL
Short Description:	This human STAT4 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	STAT4
Gene ID:	6775
Uniprot ID:	STAT4_HUMAN
Specificity:	This kit recognizes Human STAT4 in samples. No significant cross-reactivity or interference between Human STAT4 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Acetylation at Lys-667 is required for JAK2-mediated phosphorylation and activation of STAT4. Tyrosine phosphorylated upon IL12 and IFN-alpha activation, but not by IFN-gamma in T-lymphocytes and NK cells. Serine phosphorylation is required for maximal transcriptional activity but not for DNA binding. Phosphorylation by MAP2K6 at Ser-721 is required for full transcriptional activity induced by IL12. However this serine phosphorylation is not required for cell proliferation although critical for IFN-gamma production.
Function	Transcriptional regulator mainly expressed in hematopoietic cells that plays a critical role in cellular growth, differentiation and immune response. Plays a key role in the differentiation of T-helper 1 cells and the production of interferon-gamma. Participates also in multiple neutrophil functions including chemotaxis and production of the neutrophil extracellular traps. After IL12 binding to its receptor IL12RB2, STAT4 interacts with the intracellular domain of IL12RB2 and becomes tyrosine phosphorylated. Phosphorylated STAT4 then homodimerizes and migrates to the nucleus where it can recognize STAT target sequences present in IL12 responsive genes. Although IL12 appears to be the predominant activating signal, STAT4 can also be phosphorylated and activated in response to IFN-gamma stimulation via JAK1 and TYK2 and in response to different interleukins including IL23, IL2 and IL35. Transcription activation of IFN-gamma gene is mediated by interaction with JUN that forms a complex that efficiently interacts with the AP-1-related sequence of the IFN-gamma promoter. In response to IFN-alpha/beta signaling, acts as a transcriptional repressor and suppresses IL5 and IL13 mRNA expression during response to T-cell receptor (TCR) activation.
Protein Name	Signal Transducer And Activator Of Transcription 4
Database Links	Reactome: R-HSA-8854691 Reactome: R-HSA-8950505 Reactome: R-HSA-8984722 Reactome: R-HSA-9020591 Reactome: R-HSA-9020933 Reactome: R-HSA-9020958
Cellular Localisation	Cytoplasm Nucleus Translocated Into The Nucleus In Response To Phosphorylation
Alternative CLIA Names	Signal Transducer And Activator Of Transcription 4 CLIA kit STAT4 CLIA kit
Specificity	This kit recognizes Human STAT4 in samples. No significant cross-reactivity or interference between Human STAT4 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	95-110	103
EDTA plasma(n=8)	91-105	97
Cell culture media(n=8)	90-104	97

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	187.61	493.57	1945.38	180.49	498.78	1786.91
Standard deviation	22.63	56.17	204.65	18.25	38.01	186.55
CV (%)	12.06	11.38	10.52	10.11	7.62	10.44

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human STAT4 (Signal transducer and activator of transcription 4) CLIA Kit (STJC0000895)