Human TGFBI (Transforming growth factor-beta-induced protein ig-h3) CLIA Kit (STJC0000744) STJC0000744 上海玉博生物科技有限公司

Human TGFBI (Transforming growth factor-beta-induced protein ig-h3) CLIA Kit (STJC0000744)

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产品名称：Human TGFBI (Transforming growth factor-beta-induced protein ig-h3) CLIA Kit (STJC0000744)
产品型号：STJC0000744
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human TGFBI (Transforming growth factor-beta-induced protein ig-h3) CLIA Kit (STJC0000744)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	75pg/mL
Detection Limit:	125~8000pg/mL
Short Description:	This human TGFBI kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	TGFBI
Gene ID:	7045
Uniprot ID:	BGH3_HUMAN
Specificity:	This kit recognizes Human TGF Beta I in samples. No significant cross-reactivity or interference between Human TGF Beta I and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Highly expressed in the corneal epithelium. Expressed in heart, placenta, lung, liver, skeletal muscle, kidney and pancreas.
Post Translational Modifications	Gamma-carboxylation is controversial. Gamma-carboxyglutamated.gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation.these residues may be required for binding to calcium. According to a more recent report, does not contain vitamin K-dependent gamma-carboxyglutamate residues. The EMI domain contains 2 expected intradomain disulfide bridges (Cys-49-Cys85 and Cys-84-Cys-97) and one unusual interdomain disulfide bridge to the second FAS1 domain (Cys-74-Cys-339). This arrangement violates the predicted disulfide bridge pattern of an EMI domain.
Function	Plays a role in cell adhesion. May play a role in cell-collagen interactions.
Protein Name	Transforming Growth Factor-Beta-Induced Protein Ig-H3 Beta Ig-H3 Kerato-Epithelin Rgd-Containing Collagen-Associated Protein Rgd-Cap
Database Links	Reactome: R-HSA-977225
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix May Be Associated Both With Microfibrils And With The Cell Surface
Alternative CLIA Names	Transforming Growth Factor-Beta-Induced Protein Ig-H3 CLIA kit Beta Ig-H3 CLIA kit Kerato-Epithelin CLIA kit Rgd-Containing Collagen-Associated Protein CLIA kit Rgd-Cap CLIA kit TGFBI CLIA kit BIGH3 CLIA kit
Specificity	This kit recognizes Human TGFβI in samples. No significant cross-reactivity or interference between Human TGFβI and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	87-100	92
EDTA plasma(n=8)	98-112	106
Cell culture media(n=8)	89-100	95

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	425.31	829.69	3176.19	448.82	895.81	3347.96
Standard deviation	41.08	91.76	365.26	51.17	65.04	264.49
CV (%)	9.66	11.06	11.50	11.40	7.26	7.90

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human TGFBI (Transforming growth factor-beta-induced protein ig-h3) CLIA Kit (STJC0000744)