Human TNF (Tumor necrosis factor) CLIA Kit (STJC0000099) STJC0000099 上海玉博生物科技有限公司

如果您对该产品感兴趣的话，可以
产品名称：Human TNF (Tumor necrosis factor) CLIA Kit (STJC0000099)
产品型号：STJC0000099
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human TNF (Tumor necrosis factor) CLIA Kit (STJC0000099)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	4.69pg/mL
Detection Limit:	7.81~500pg/mL
Short Description:	This human TNF kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	TNF
Gene ID:	7124
Uniprot ID:	TNFA_HUMAN
Specificity:	This kit recognizes Human TNF-Alpha in samples. No significant cross-reactivity or interference between Human TNF-Alpha and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	The soluble form derives from the membrane form by proteolytic processing. The membrane-bound form is further proteolytically processed by SPPL2A or SPPL2B through regulated intramembrane proteolysis producing TNF intracellular domains (ICD1 and ICD2) released in the cytosol and TNF C-domain 1 and C-domain 2 secreted into the extracellular space. The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1. O-glycosylated.glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid. Tumor necrosis factor, soluble form: The soluble form is demyristoylated at Lys-19 and Lys-20 by SIRT6, promoting its secretion.
Function	Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Impairs regulatory T-cells (Treg) function in individuals with rheumatoid arthritis via FOXP3 dephosphorylation. Up-regulates the expression of protein phosphatase 1 (PP1), which dephosphorylates the key 'Ser-418' residue of FOXP3, thereby inactivating FOXP3 and rendering Treg cells functionally defective. Key mediator of cell death in the anticancer action of BCG-stimulated neutrophils in combination with DIABLO/SMAC mimetic in the RT4v6 bladder cancer cell line. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6. Promotes osteoclastogenesis and therefore mediates bone resorption. The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
Protein Name	Tumor Necrosis Factor Cachectin Tnf-Alpha Tumor Necrosis Factor Ligand Superfamily Member 2 Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form N-Terminal Fragment Ntf - Intracellular Domain 1 Icd1 - Intracellular Domain 2 Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form
Database Links	Reactome: R-HSA-381340 Reactome: R-HSA-5357786 Reactome: R-HSA-5357905 Reactome: R-HSA-5357956 Reactome: R-HSA-5626978 Reactome: R-HSA-5668541 Reactome: R-HSA-6783783 Reactome: R-HSA-6785807 Reactome: R-HSA-75893
Cellular Localisation	Cell Membrane Single-Pass Type Ii Membrane Protein Tumor Necrosis Factor Membrane Form: Membrane Soluble Form: Secreted C-Domain 1: Secreted C-Domain 2: Secreted
Alternative CLIA Names	Tumor Necrosis Factor CLIA kit Cachectin CLIA kit Tnf-Alpha CLIA kit Tumor Necrosis Factor Ligand Superfamily Member 2 CLIA kit Tnf-A Cleaved Into - Tumor Necrosis Factor - Membrane Form CLIA kit N-Terminal Fragment CLIA kit Ntf - Intracellular Domain 1 CLIA kit Icd1 - Intracellular Domain 2 CLIA kit Icd2 - C-Domain 1 - C-Domain 2 - Tumor Necrosis Factor - Soluble Form CLIA kit TNF CLIA kit TNFA CLIA kit TNFSF2 CLIA kit
Specificity	This kit recognizes Human TNF-α in samples. No significant cross-reactivity or interference between Human TNF-α and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	100-113	107
EDTA plasma(n=8)	92-103	98
Cell culture media(n=8)	87-101	92

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	25.60	58.03	169.01	27.58	54.45	158.50
Standard deviation	2.69	5.50	13.54	2.46	6.40	14.99
CV (%)	10.51	9.48	8.01	8.92	11.75	9.46

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

产品留言

标题

联系人

联系电话

内容

验证码

点击换一张

注：1.可以使用快捷键Alt+S或Ctrl+Enter发送信息!
2.如有必要,请您留下您的详细联系方式!

温馨提示：为规避购买风险，建议您在购买前务必确认供应商资质与产品质量。
免责申明：以上内容为注册会员自行发布，若信息的真实性、合法性存在争议，平台将会监督协助处理，欢迎举报

产品目录

产品资料

Human TNF (Tumor necrosis factor) CLIA Kit (STJC0000099)