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Human TNFSF10 (Tumor necrosis factor ligand superfamily member 10) CLIA Kit (STJC0000721)

Human TNFSF10 (Tumor necrosis factor ligand superfamily member 10) CLIA Kit (STJC0000721)
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  • 产品名称:Human TNFSF10 (Tumor necrosis factor ligand superfamily member 10) CLIA Kit (STJC0000721)
  • 产品型号:STJC0000721
  • 产品展商:ST john's laboratory
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简单介绍
Human TNFSF10 (Tumor necrosis factor ligand superfamily member 10) CLIA Kit (STJC0000721)
产品描述
Applications: CLIA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 4.69pg/mL
Detection Limit: 7.81~500pg/mL
Short Description: This human TNFSF10 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Chemiluminescence
Gene Symbol: TNFSF10
Gene ID: 8743
Uniprot ID: TNF10_HUMAN
Specificity: This kit recognizes Human TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Human TRAIL/TNFSF10 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Tissue Specificity Widespread.most predominant in spleen, lung and prostate.
Post Translational Modifications Tyrosine phosphorylated by PKDCC/VLK.
Function Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG. Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
Protein Name Tumor Necrosis Factor Ligand Superfamily Member 10
Apo-2 Ligand
Apo-2l
Tnf-Related Apoptosis-Inducing Ligand
Protein Trail
Cd Antigen Cd253
Database Links Reactome: R-HSA-140534
Reactome: R-HSA-3371378
Reactome: R-HSA-5213460
Reactome: R-HSA-5218900
Reactome: R-HSA-69416
Reactome: R-HSA-75158
Cellular Localisation Cell Membrane
Single-Pass Type Ii Membrane Protein
Secreted
Exists Both As Membrane-Bound And Soluble Form
Alternative CLIA Names Tumor Necrosis Factor Ligand Superfamily Member 10 CLIA kit
Apo-2 Ligand CLIA kit
Apo-2l CLIA kit
Tnf-Related Apoptosis-Inducing Ligand CLIA kit
Protein Trail CLIA kit
Cd Antigen Cd253 CLIA kit
TNFSF10 CLIA kit
APO2L CLIA kit
TRAIL CLIA kit
Specificity This kit recognizes Human TRAIL/TNFSF10 in samples. No significant cross-reactivity or interference between Human TRAIL/TNFSF10 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro CLIA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent A 1 vial, 5 mL 2-8℃ (Protect from light)
Substrate Reagent B 1 vial, 5 mL 2-8℃ (Protect from light)
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 93-106 100
EDTA plasma(n=8) 93-109 100
Cell culture media(n=8) 95-111 101

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 25.76 79.38 247.65 25.13 83.06 237.53
Standard deviation 2.74 8.76 16.00 2.70 6.65 26.94
CV (%) 10.64 11.04 6.46 10.74 8.01 11.34
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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