Human TNFSF12 (Tumor necrosis factor ligand superfamily member 12) Sandwich ELISA (STJE0003799) STJE0003799 上海玉博生物科技有限公司

Human TNFSF12 (Tumor necrosis factor ligand superfamily member 12) Sandwich ELISA (STJE0003799)

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产品名称：Human TNFSF12 (Tumor necrosis factor ligand superfamily member 12) Sandwich ELISA (STJE0003799)
产品型号：STJE0003799
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human TNFSF12 (Tumor necrosis factor ligand superfamily member 12) Sandwich ELISA (STJE0003799)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	4.69pg/mL
Detection Limit:	7.81~500pg/mL
Short Description:	This TNFSF12 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	TNFSF12
Gene ID:	407977/8742
Uniprot ID:	TNF12_HUMAN
Specificity:	This kit recognizes Human TWEAK in samples. No significant cross-reactivity or interference between Human TWEAK and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	The soluble form derives from the membrane form by proteolytic processing.
Function	Binds to FN14 and possibly also to TNRFSF12/APO3. Weak inducer of apoptosis in some cell types. Mediates NF-kappa-B activation. Promotes angiogenesis and the proliferation of endothelial cells. Also involved in induction of inflammatory cytokines. Promotes IL8 secretion.
Protein Name	Tumor Necrosis Factor Ligand Superfamily Member 12 Apo3 Ligand Tnf-Related Weak Inducer Of Apoptosis Tweak Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 12 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 12 - Secreted Form
Database Links	Reactome: R-HSA-5668541 Reactome: R-HSA-5676594
Cellular Localisation	Cell Membrane Single-Pass Type Ii Membrane Protein Tumor Necrosis Factor Ligand Superfamily Member 12 Secreted Form: Secreted Isoform Twe-Pril: Cell Membrane Single-Pass Membrane Protein
Alternative ELISA Names	Tumor Necrosis Factor Ligand Superfamily Member 12 ELISA kit Apo3 Ligand ELISA kit Tnf-Related Weak Inducer Of Apoptosis ELISA kit Tweak Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 12 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 12 - Secreted Form ELISA kit TNFSF12 ELISA kit APO3L ELISA kit DR3LG ELISA kit UNQ181 ELISA kit PRO207 ELISA kit
Specificity	This kit recognizes Human TWEAK in samples. No significant cross-reactivity or interference between Human TWEAK and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	99-110	105
EDTA plasma (n=8)	96-107	102
Cell culture media (n=8)	87-98	94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	20.42	60.19	223.79	22.00	53.54	200.93
Standard deviation	1.06	2.88	11.70	1.31	2.66	9.50
CV (%)	5.19	4.78	5.23	5.95	4.97	4.73

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human TNFSF12 (Tumor necrosis factor ligand superfamily member 12) Sandwich ELISA (STJE0003799)