Human TNFSF14 (Tumor necrosis factor ligand superfamily member 14) CLIA Kit (STJC0000086) STJC0000086 上海玉博生物科技有限公司

Human TNFSF14 (Tumor necrosis factor ligand superfamily member 14) CLIA Kit (STJC0000086)

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产品名称：Human TNFSF14 (Tumor necrosis factor ligand superfamily member 14) CLIA Kit (STJC0000086)
产品型号：STJC0000086
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human TNFSF14 (Tumor necrosis factor ligand superfamily member 14) CLIA Kit (STJC0000086)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	7.5pg/mL
Detection Limit:	12.5~800pg/mL
Short Description:	This human TNFSF14 kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	TNFSF14
Gene ID:	8740
Uniprot ID:	TNF14_HUMAN
Specificity:	This kit recognizes Human TNFSF14 in samples. No significant cross-reactivity or interference between Human TNFSF14 and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Predominantly expressed in the spleen but also found in the brain. Weakly expressed in peripheral lymphoid tissues and in heart, placenta, liver, lung, appendix, and kidney, and no expression seen in fetal tissues, endocrine glands, or nonhematopoietic tumor lines.
Post Translational Modifications	N-glycosylated. The soluble form of isoform 1 derives from the membrane form by proteolytic processing.
Function	Cytokine that binds to TNFRSF3/LTBR. Binding to the decoy receptor TNFRSF6B modulates its effects. Acts as a ligand for TNFRSF14/HVEM. Upon binding to TNFRSF14/HVEM, delivers costimulatory signals to T cells, leading to T cell proliferation and IFNG production.
Protein Name	Tumor Necrosis Factor Ligand Superfamily Member 14 Herpes Virus Entry Mediator Ligand Hvem-L Herpesvirus Entry Mediator Ligand Cd Antigen Cd258 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 14 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 14 - Soluble Form
Database Links	Reactome: R-HSA-5668541 Reactome: R-HSA-5669034 Reactome: R-HSA-5676594
Cellular Localisation	Tumor Necrosis Factor Ligand Superfamily Member 14 Membrane Form: Cell Membrane Single-Pass Type Ii Membrane Protein Soluble Form: Secreted Isoform 2: Cytoplasm
Alternative CLIA Names	Tumor Necrosis Factor Ligand Superfamily Member 14 CLIA kit Herpes Virus Entry Mediator Ligand CLIA kit Hvem-L CLIA kit Herpesvirus Entry Mediator Ligand CLIA kit Cd Antigen Cd258 Cleaved Into - Tumor Necrosis Factor Ligand Superfamily Member 14 - Membrane Form - Tumor Necrosis Factor Ligand Superfamily Member 14 - Soluble Form CLIA kit TNFSF14 CLIA kit HVEML CLIA kit LIGHT CLIA kit UNQ391 CLIA kit PRO726 CLIA kit
Specificity	This kit recognizes Human TNFSF14 in samples. No significant cross-reactivity or interference between Human TNFSF14 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	86-98	93
EDTA plasma(n=8)	93-107	101
Cell culture media(n=8)	89-100	94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	43.42	78.25	311.28	43.07	75.80	315.52
Standard deviation	5.31	7.90	23.69	4.67	6.24	21.77
CV (%)	12.23	10.10	7.61	10.84	8.23	6.90

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human TNFSF14 (Tumor necrosis factor ligand superfamily member 14) CLIA Kit (STJC0000086)