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Human TNNT2 (Troponin T, cardiac muscle) Sandwich ELISA (STJE0002370)

Human TNNT2 (Troponin T, cardiac muscle) Sandwich ELISA (STJE0002370)
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  • 产品名称:Human TNNT2 (Troponin T, cardiac muscle) Sandwich ELISA (STJE0002370)
  • 产品型号:STJE0002370
  • 产品展商:ST john's laboratory
  • 产品文档:无相关文档
简单介绍
Human TNNT2 (Troponin T, cardiac muscle) Sandwich ELISA (STJE0002370)
产品描述
Applications: ELISA
Reactivity: Human
Note: FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity: 9.38pg/mL
Detection Limit: 15.63~1000pg/mL
Short Description: This TNNT2 Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction: If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time: 3.5h
Detection: Colormetric
Gene Symbol: TNNT2
Gene ID: 7139
Uniprot ID: TNNT2_HUMAN
Specificity: This kit recognizes Human cTnT/TNNT2 in samples. No significant cross-reactivity or interference between Human cTnT/TNNT2 and analogues was observed.
Sample Type: Serum, plasma and other biological fluids
Post Translational Modifications Phosphorylation at Thr-213 by PRKCA induces significant reduction in myofilament calcium sensitivity and actomyosin ATPase activity.
Function Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Protein Name Troponin T - Cardiac Muscle
Tntc
Cardiac Muscle Troponin T
Ctnt
Database Links Reactome: R-HSA-390522
Alternative ELISA Names Troponin T - Cardiac Muscle ELISA kit
Tntc ELISA kit
Cardiac Muscle Troponin T ELISA kit
Ctnt ELISA kit
TNNT2 ELISA kit
Specificity This kit recognizes Human cTnT/TNNT2 in samples. No significant cross-reactivity or interference between Human cTnT/TNNT2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are

Information sourced from Uniprot.org


Item Specifications Storage
Micro ELISA Plate (Dismountable) 96T: 8 wells ×12 strips strips -20℃, 6 months
Reference Standard 96T: 2 vials 48T: 1 vial -20℃, 6 months
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL 60 μL -20℃, 6 months
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL 60 μL -20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL 2-8°C, 6 months
HRP Conjugate Diluent 1 vial, 14 mL 2-8°C, 6 months
Concentrated Wash Buffer (25×) 1 vial, 30 mL 2-8°C, 6 months
Substrate Reagent 1 vial, 10 mL 2-8℃ (Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-102 94
EDTA plasma (n=8) 93-104 98
Cell culture media (n=8) 91-105 98

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample 1.00 2.00 3.00 1.00 2.00 3.00
n 20.00 20.00 20.00 20.00 20.00 20.00
Mean (pg/mL) 45.94 100.24 399.56 47.33 99.68 413.65
Standard deviation 2.79 4.31 18.30 2.49 4.39 17.79
CV (%) 6.07 4.30 4.58 5.26 4.40 4.30
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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