Human TOP1 (Topoisomerase I) Sandwich ELISA Kit Ready-To-Use (STJE0019120) STJE0019120 上海玉博生物科技有限公司

Human TOP1 (Topoisomerase I) Sandwich ELISA Kit Ready-To-Use (STJE0019120)

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产品名称：Human TOP1 (Topoisomerase I) Sandwich ELISA Kit Ready-To-Use (STJE0019120)
产品型号：STJE0019120
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human TOP1 (Topoisomerase I) Sandwich ELISA Kit Ready-To-Use (STJE0019120)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Sensitivity:	0.60ng/mL
Detection Limit:	1.56-100ng/mL
Short Description:	This TOP1 Sandwich ELISA Kit, Ready-To-Use is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	The whole kit may be stored at-20°C for up to 12 months from receipt. An unopened kit may be stored in the fridge at 2-8°C for up to 6 months. Once opened store individual kit contents according to components table provided with the kit.
Assay Time:	3 hrs

Gene Symbol:	TOP1
Gene ID:	7150
Uniprot ID:	TOP1_HUMAN
Immunogen Region:	Ready-To-Use
Sample Type:	tissue homogenates, cell lysates or other biological fluids.

Tissue Specificity	Endothelial cells.
Post Translational Modifications	Sumoylated. Lys-117 is the main site of sumoylation. Sumoylation plays a role in partitioning TOP1 between nucleoli and nucleoplasm. Levels are dramatically increased on camptothecin (CPT) treatment. Phosphorylation at Ser-506 by CK2 increases binding to supercoiled DNA and sensitivity to camptothecin.
Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then rotates around the intact phosphodiester bond on the opposing strand, thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Regulates the alternative splicing of tissue factor (F3) pre-mRNA in endothelial cells. Involved in the circadian transcription of the core circadian clock component BMAL1 by altering the chromatin structure around the ROR response elements (ROREs) on the BMAL1 promoter.
Protein Name	Dna Topoisomerase 1 Dna Topoisomerase I
Database Links	Reactome: R-HSA-4615885
Cellular Localisation	Nucleus Nucleolus Nucleoplasm Diffuse Nuclear Localization With Some Enrichment In Nucleoli On Cpt Treatment Cleared From Nucleoli Into Nucleoplasm Sumoylated Forms Found In Both Nucleoplasm And Nucleoli
Alternative ELISA Names	Dna Topoisomerase 1 ELISA kit Dna Topoisomerase I ELISA kit TOP1 ELISA kit
output

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human TOP1 (Topoisomerase I) Sandwich ELISA Kit Ready-To-Use (STJE0019120)