Human VCAN (Versican core protein) Sandwich ELISA (STJE0002814) STJE0002814 上海玉博生物科技有限公司

Human VCAN (Versican core protein) Sandwich ELISA (STJE0002814)

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产品名称：Human VCAN (Versican core protein) Sandwich ELISA (STJE0002814)
产品型号：STJE0002814
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human VCAN (Versican core protein) Sandwich ELISA (STJE0002814)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	0.19ng/mL
Detection Limit:	0.31~20ng/mL
Short Description:	This VCAN Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	VCAN
Gene ID:	1462
Uniprot ID:	CSPG2_HUMAN
Specificity:	This kit recognizes Human VCAN in samples. No significant cross-reactivity or interference between Human VCAN and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Phosphorylated by FAM20C in the extracellular medium. Proteolytically cleaved by ADAMTS5 and ADAMTS15 in the pericellular matrix surrounding myoblasts, facilitating myoblast contact and fusion which is required for skeletal muscle development and regeneration.
Function	May play a role in intercellular signaling and in connecting cells with the extracellular matrix. May take part in the regulation of cell motility, growth and differentiation. Binds hyaluronic acid.
Protein Name	Versican Core Protein Chondroitin Sulfate Proteoglycan Core Protein 2 Chondroitin Sulfate Proteoglycan 2 Glial Hyaluronate-Binding Protein Ghap Large Fibroblast Proteoglycan Pg-M
Database Links	Reactome: R-HSA-1971475 Reactome: R-HSA-2022870 Reactome: R-HSA-2022923 Reactome: R-HSA-2024101 Reactome: R-HSA-3000178 Reactome: R-HSA-3560783 Reactome: R-HSA-3560801 Reactome: R-HSA-3595172 Reactome: R-HSA-3595174 Reactome: R-HSA-3595177 Reactome: R-HSA-381426 Reactome: R-HSA-4420332 Reactome: R-HSA-8957275
Cellular Localisation	Secreted Extracellular Space Extracellular Matrix Cell Projection Cilium Photoreceptor Outer Segment Interphotoreceptor Matrix
Alternative ELISA Names	Versican Core Protein ELISA kit Chondroitin Sulfate Proteoglycan Core Protein 2 ELISA kit Chondroitin Sulfate Proteoglycan 2 ELISA kit Glial Hyaluronate-Binding Protein ELISA kit Ghap ELISA kit Large Fibroblast Proteoglycan ELISA kit Pg-M ELISA kit VCAN ELISA kit CSPG2 ELISA kit
Specificity	This kit recognizes Human VCAN in samples. No significant cross-reactivity or interference between Human VCAN and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	85-98	92
EDTA plasma (n=8)	92-104	98
Cell culture media (n=8)	88-101	94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	0.92	2.97	8.70	0.87	3.16	9.15
Standard deviation	0.05	0.13	0.39	0.06	0.17	0.38
CV (%)	5.43	4.38	4.48	6.90	5.38	4.15

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human VCAN (Versican core protein) Sandwich ELISA (STJE0002814)