Human VEGFA (Vascular endothelial growth factor A) Sandwich ELISA (STJE0002001) STJE0002001 上海玉博生物科技有限公司

Human VEGFA (Vascular endothelial growth factor A) Sandwich ELISA (STJE0002001)

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产品名称：Human VEGFA (Vascular endothelial growth factor A) Sandwich ELISA (STJE0002001)
产品型号：STJE0002001
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human VEGFA (Vascular endothelial growth factor A) Sandwich ELISA (STJE0002001)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	18.75pg/mL
Detection Limit:	31.25~2000pg/mL
Short Description:	This VEGFA Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	VEGFA
Gene ID:	7422
Uniprot ID:	VEGFA_HUMAN
Specificity:	This kit recognizes Human VEGF-A in samples. No significant cross-reactivity or interference between Human VEGF-A and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Vascular endothelial growth factor A, long form: Produced by use of an alternative upstream CUG codon and post-translationally processed into the N-terminal N-VEGF form and the C-terminal secreted VEGFA form.
Function	N-VEGF: Participates in the induction of key genes involved in the response to hypoxia and in the induction of angiogenesis such as HIF1A. Involved in protecting cells from hypoxia-mediated cell death. VEGFA: Growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. Induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Binds to the NRP1/neuropilin-1 receptor. Binding to NRP1 initiates a signaling pathway needed for motor neuron axon guidance and cell body migration, including for the caudal migration of facial motor neurons from rhombomere 4 to rhombomere 6 during embryonic development. Also binds the DEAR/FBXW7-AS1 receptor. Isoform VEGF165B: Binds to the KDR receptor but does not activate downstream signaling pathways, does not activate angiogenesis and inhibits tumor growth.
Protein Name	Vascular Endothelial Growth Factor A - Long Form L-Vegf Vascular Permeability Factor Vpf Cleaved Into - N-Vegf - Vegfa
Database Links	Reactome: R-HSA-114608 Reactome: R-HSA-1234158 Reactome: R-HSA-194138 Reactome: R-HSA-194313 Reactome: R-HSA-195399 Reactome: R-HSA-4420097 P15692-4 Reactome: R-HSA-5218921 P15692-4 Reactome: R-HSA-6785807 Reactome: R-HSA-8866910 Reactome: R-HSA-9679191 P15692-4
Cellular Localisation	N-Vegf: Cytoplasm Nucleus Cytoplasmic In Normoxic Conditions And Localizes To The Nucleus Under Hypoxic Conditions Vegfa: Secreted Isoform L-Vegf189: Endoplasmic Reticulum Golgi Apparatus Secreted Extracellular Space Extracellular Matrix Isoform Vegf121: Secreted Isoform Vegf165: Secreted Isoform Vegf189: Secreted Cell-Associated After Secretion And Is Bound Avidly By Heparin And The Extracellular Matrix Although It May Be Released As A Soluble Form By Heparin Heparinase Or Plasmin
Alternative ELISA Names	Vascular Endothelial Growth Factor A - Long Form ELISA kit L-Vegf ELISA kit Vascular Permeability Factor ELISA kit Vpf Cleaved Into - N-Vegf - Vegfa ELISA kit VEGFA ELISA kit VEGF ELISA kit
Specificity	This kit recognizes Human VEGF-A in samples. No significant cross-reactivity or interference between Human VEGF-A and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	92-105	98
EDTA plasma (n=8)	95-110	101
Cell culture media (n=8)	88-103	96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	94.10	279.80	737.20	92.40	303.90	745.00
Standard deviation	5.50	14.30	34.60	4.80	15.50	31.30
CV (%)	5.84	5.11	4.69	5.19	5.10	4.20

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human VEGFA (Vascular endothelial growth factor A) Sandwich ELISA (STJE0002001)