Human VIM (Vimentin) CLIA Kit (STJC0000518) STJC0000518 上海玉博生物科技有限公司

Human VIM (Vimentin) CLIA Kit (STJC0000518)

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产品名称：Human VIM (Vimentin) CLIA Kit (STJC0000518)
产品型号：STJC0000518
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human VIM (Vimentin) CLIA Kit (STJC0000518)

产品描述

Applications:	CLIA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	37.5pg/mL
Detection Limit:	62.5~4000pg/mL
Short Description:	This human VIM kit is a highly sensitive in-vitro chemiluminescent immunoassay for the measurement of trace amounts of analytes.

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Chemiluminescence

Gene Symbol:	VIM
Gene ID:	7431
Uniprot ID:	VIME_HUMAN
Specificity:	This kit recognizes Human VIM in samples. No significant cross-reactivity or interference between Human VIM and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Tissue Specificity	Highly expressed in fibroblasts, some expression in T- and B-lymphocytes, and little or no expression in Burkitt's lymphoma cell lines. Expressed in many hormone-independent mammary carcinoma cell lines.
Post Translational Modifications	Filament disassembly during mitosis is promoted by phosphorylation at Ser-55 as well as by nestin. One of the most prominent phosphoproteins in various cells of mesenchymal origin. Phosphorylation is enhanced during cell division, at which time vimentin filaments are significantly reorganized. Phosphorylation by PKN1 inhibits the formation of filaments. Phosphorylated at Ser-56 by CDK5 during neutrophil secretion in the cytoplasm. Phosphorylated by STK33. Phosphorylated on tyrosine residues by SRMS. O-glycosylated during cytokinesis at sites identical or close to phosphorylation sites, this interferes with the phosphorylation status. S-nitrosylation is induced by interferon-gamma and oxidatively-modified low-densitity lipoprotein (LDL(ox)) possibly implicating the iNOS-S100A8/9 transnitrosylase complex.
Function	Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
Protein Name	Vimentin
Database Links	Reactome: R-HSA-264870 Reactome: R-HSA-390522 Reactome: R-HSA-6785807 Reactome: R-HSA-9013422 Reactome: R-HSA-9613829 Reactome: R-HSA-9615710 Reactome: R-HSA-9646399
Cellular Localisation	Cytoplasm Cytoskeleton Nucleus Matrix Cell Membrane
Alternative CLIA Names	Vimentin CLIA kit VIM CLIA kit
Specificity	This kit recognizes Human VIM in samples. No significant cross-reactivity or interference between Human VIM and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro CLIA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent A	1 vial, 5 mL	2-8℃ (Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃ (Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	101-114	107
EDTA plasma(n=8)	91-104	96
Cell culture media(n=8)	88-105	96

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	208.16	343.52	1564.42	189.76	341.33	1570.91
Standard deviation	18.55	28.17	171.15	17.15	37.61	118.92
CV (%)	8.91	8.20	10.94	9.04	11.02	7.57

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human VIM (Vimentin) CLIA Kit (STJC0000518)