Human VLDLR (Very low-density lipoprotein receptor) Sandwich ELISA (STJE0002995) STJE0002995 上海玉博生物科技有限公司

Human VLDLR (Very low-density lipoprotein receptor) Sandwich ELISA (STJE0002995)

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产品名称：Human VLDLR (Very low-density lipoprotein receptor) Sandwich ELISA (STJE0002995)
产品型号：STJE0002995
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Human VLDLR (Very low-density lipoprotein receptor) Sandwich ELISA (STJE0002995)

产品描述

Applications:	ELISA
Reactivity:	Human
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	1.88ng/mL
Detection Limit:	3.13~200ng/mL
Short Description:	This VLDLR Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in human cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	3.5h
Detection:	Colormetric

Gene Symbol:	VLDLR
Gene ID:	7436
Uniprot ID:	VLDLR_HUMAN
Specificity:	This kit recognizes Human VLDLR in samples. No significant cross-reactivity or interference between Human VLDLR and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	Ubiquitinated at Lys-839 by MYLIP leading to degradation. Glycosylated.
Function	Multifunctional cell surface receptor that binds VLDL and transports it into cells by endocytosis and therefore plays an important role in energy metabolism. Binds also to a wide range of other molecules including Reelin/RELN or apolipoprotein E/APOE-containing ligands as well as clusterin/CLU. In the off-state of the pathway, forms homooligomers or heterooligomers with LRP8. Upon binding to ligands, homooligomers are rearranged to higher order receptor clusters that transmit the extracellular RELN signal to intracellular signaling processes by binding to DAB1. This interaction results in phosphorylation of DAB1 leading to the ultimate cell responses required for the correct positioning of newly generated neurons. Later, mediates a stop signal for migrating neurons, preventing them from entering the marginal zone. (Microbial infection) Acts as a receptor for Semliki Forest virus.
Protein Name	Very Low-Density Lipoprotein Receptor Vldl Receptor Vldl-R
Database Links	Reactome: R-HSA-8866376 Reactome: R-HSA-8866427 Reactome: R-HSA-8964046
Cellular Localisation	Cell Membrane Single-Pass Type I Membrane Protein Membrane Clathrin-Coated Pit
Alternative ELISA Names	Very Low-Density Lipoprotein Receptor ELISA kit Vldl Receptor ELISA kit Vldl-R ELISA kit VLDLR ELISA kit
Specificity	This kit recognizes Human VLDLR in samples. No significant cross-reactivity or interference between Human VLDLR and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	92-106	100
EDTA plasma (n=8)	90-102	96
Cell culture media (n=8)	87-103	94

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (ng/mL)	10.88	30.87	89.76	11.78	28.38	90.90
Standard deviation	0.70	1.49	3.46	0.64	1.26	4.67
CV (%)	6.43	4.83	3.85	5.43	4.44	5.14

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Human VLDLR (Very low-density lipoprotein receptor) Sandwich ELISA (STJE0002995)