Mouse Sst (Somatostatin) Competitive ELISA (STJE0004990) STJE0004990 上海玉博生物科技有限公司

Mouse Sst (Somatostatin) Competitive ELISA (STJE0004990)

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产品名称：Mouse Sst (Somatostatin) Competitive ELISA (STJE0004990)
产品型号：STJE0004990
产品展商：ST john's laboratory
产品文档：无相关文档

简单介绍

Mouse Sst (Somatostatin) Competitive ELISA (STJE0004990)

产品描述

Applications:	ELISA
Reactivity:	Mouse
Note:	FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:	4.69pg/mL
Detection Limit:	7.81~500pg/mL
Short Description:	This Sst Competitive ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in mouse cell culture supernatant, serum and plasma (EDTA, citrate, heparin).

Storage Instruction:	If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:	2.5h
Detection:	Colormetric

Gene Symbol:	Sst
Gene ID:	20604
Uniprot ID:	SMS_MOUSE
Specificity:	This kit recognizes Mouse SST in samples. No significant cross-reactivity or interference between Mouse SST and analogues was observed.
Sample Type:	Serum, plasma and other biological fluids

Post Translational Modifications	C-terminal amidation of the neuronostatin peptide is required for its biological activity, including for the regulation of mean arterial pressure.
Function	Somatostatin-14: Inhibits the secretion of pituitary hormones, including that of growth hormone/somatotropin (GH1), PRL, ACTH, luteinizing hormone (LH) and TSH. Also impairs ghrelin- and GnRH-stimulated secretion of GH1 and LH.the inhibition of ghrelin-stimulated secretion of GH1 can be further increased by neuronostatin. Neuronostatin: May enhance low-glucose-induced glucagon release by pancreatic alpha cells. This effect may be mediated by binding to GPR107 and PKA activation. May regulate cardiac contractile function. May compromise cardiomyocyte viability. In the central nervous system, may impair memory retention and may affect hippocampal excitability. May also have anxiolytic and anorexigenic effects. May play a role in arterial pressure regulation. May inhibit basal, but not ghrelin- or GnRH-stimulated secretion of GH1 or LH, but does not affect the release of other pituitary hormones, including PRL, ACTH, FSH or TSH. Potentiates inhibitory action of somatostatin on ghrelin-stimulated secretion of GH1, but not that on GnRH-stimulated secretion of LH.
Protein Name	Somatostatin Cleaved Into - Antrin - Somatostatin-28 - Somatostatin-14 - Neuronostatin Nst
Database Links	Reactome: R-MMU-375276 Reactome: -MMU-418594
Cellular Localisation	Secreted
Alternative ELISA Names	Somatostatin Cleaved Into - Antrin - Somatostatin-28 - Somatostatin-14 - Neuronostatin ELISA kit Nst ELISA kit Sst ELISA kit Smst ELISA kit
Specificity	This kit recognizes SST in samples. No significant cross-reactivity or interference between SST and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are

Information sourced from Uniprot.org

Item Specifications Storage

Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial	-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 60 μL	-20℃, 6 months
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 60 μL	-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL	2-8°C, 6 months
HRP Conjugate Diluent	1 vial, 14 mL	2-8°C, 6 months
Concentrated Wash Buffer (25×)	1 vial, 30 mL	2-8°C, 6 months
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Sample Type Range (%) Average Recovery (%)

Serum(n=8)	89-103	95
EDTA plasma(n=8)	84-97	90
Cell culture media(n=8)	86-96	91

Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision

Sample	1.00	2.00	3.00	1.00	2.00	3.00
n	20.00	20.00	20.00	20.00	20.00	20.00
Mean (pg/mL)	26.50	74.00	196.80	28.70	68.50	190.70
Standard deviation	1.40	4.10	10.20	1.50	2.70	8.40
CV (%)	5.28	5.54	5.18	5.23	3.94	4.40

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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Mouse Sst (Somatostatin) Competitive ELISA (STJE0004990)