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Porcine IL-1 Beta (Interleukin 1 Beta) Sandwich ELISA (STJE0019943)
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Porcine IL-1 Beta (Interleukin 1 Beta) Sandwich ELISA (STJE0019943)
产品资料
Porcine IL-1 Beta (Interleukin 1 Beta) Sandwich ELISA (STJE0019943)
如果您对该产品感兴趣的话,可以
产品名称:
Porcine IL-1 Beta (Interleukin 1 Beta) Sandwich ELISA (STJE0019943)
产品型号:
STJE0019943
产品展商:
ST john's laboratory
产品文档:
无相关文档
简单介绍
Porcine IL-1 Beta (Interleukin 1 Beta) Sandwich ELISA (STJE0019943)
产品描述
Applications:
ELISA
Reactivity:
Porcine
Note:
FOR SCIENTIFIC EDUCATIONAL RESEARCH USE ONLY (RUO). MUST NOT BE USED IN DIAGNOSTIC OR OTHER MEDICAL APPLICATIONS.
Sensitivity:
4.69 pg/mL
Detection Limit:
7.81-500 pg/mL
Short Description:
This IL-1 Beta Sandwich ELISA is an in-vitro enzyme-linked immunosorbent assay for the measurement of samples in porcine cell culture supernatant, serum and plasma (EDTA, citrate, heparin).
Storage Instruction:
If unopened the kit may be stored at 2-8°C for up to 1 month. If the kit will not be used within 1 month, store the components separately, according to the component table in the manual.
Assay Time:
3.5h
Detection:
Colormetric
Specificity:
This kit recognizes Porcine IL-1 Beta in samples. No significant cross-reactivity or interference between Porcine IL-1 Beta and analogues was observed.
Sample Type:
serum, plasma and other biological fluids
Specificity
This kit recognizes Porcine IL-1β in samples. No significant cross-reactivity or interference between Porcine IL-1β and analogues was observed.
Reproducibility
Both intra-CV and inter-CV are
Information sourced from Uniprot.org
Item Specifications Storage
Micro ELISA Plate (Dismountable)
96T: 8 wells ×12 strips strips
-20℃, 6 months
Reference Standard
96T: 2 vials 48T: 1 vial
-20℃, 6 months
Concentrated Biotinylated Detection Ab (100×)
96T: 1 vial, 120 μL 60 μL
-20℃, 6 months
Concentrated HRP Conjugate (100×)
96T: 1 vial, 120 μL 60 μL
-20℃ (Protect from light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
2-8°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
2-8°C, 6 months
HRP Conjugate Diluent
1 vial, 14 mL
2-8°C, 6 months
Concentrated Wash Buffer (25×)
1 vial, 30 mL
2-8°C, 6 months
Substrate Reagent
1 vial, 10 mL
2-8℃ (Protect from light)
Stop Solution
1 vial, 10 mL
2-8°C
Plate Sealer
5 pieces
Manual
1 copy
Certificate of Analysis
1 copy
Sample Type Range (%) Average Recovery (%)
Serum(n=8)
90-104
97
EDTA plasma (n=8)
92-104
98
Cell culture media (n=8)
84-97
90
Intra-assay Precision Intra-assay Precision Intra-assay Precision Inter-assay Precision Inter-assay Precision Inter-assay Precision
Sample
1.00
2.00
3.00
1.00
2.00
3.00
n
20.00
20.00
20.00
20.00
20.00
20.00
Mean (pg/mL)
23.53
67.32
229.46
21.37
71.74
236.37
Standard deviation
1.24
3.24
11.52
1.47
4.15
8.08
CV (%)
5.26
4.81
5.02
6.88
5.79
3.42
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
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