产品说明:小鼠CD19微珠用于从外周血和其它组织(如骨髓、**结和脾脏)中阳性选择或去除小鼠B细胞。CD19从早期B祖细胞开始表达,见于B细胞系发育全过程,终末分化为浆细胞时表达下调。
应用:CD19微珠分选的B细胞可用于体内印迹法(footprinting)研究B细胞发育过程中增强子的可插入性。纯化B细胞的提取物可用于在无细胞体系中分析V(D)J基因重排的条件。此外,从分选的B细胞中提取的mRNA可用于基因芯片法分析B细胞发育过程中转录因子的定量表达。
分选柱: 阳性分选:MS、LS、XS或autoMACSTM分选柱。 去除分选:LD、CS、D或autoMACS分选柱。
Isolation of mouse B cells from a spleen cell suspension using CD19 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells are fluorescently stained with CD19-FITC and CD45R (B220)-PE. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence. Unseparated fraction Isolated CD19+ cells
Isolation of mouse B cells from a spleen cell suspension using CD19 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells are fluorescently stained with CD19-FITC and CD45R (B220)-PE. Cell debris and dead cells are excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction Isolated CD19+ cells