说明:小鼠CD11c微珠用于从**和非**组织单细胞悬液中分选小鼠DCs。与人不同,在小鼠中CD11c表达于所有定义的DC亚群。用CD11c 微珠阳性分选DCs,快速而简单,取代了以往的繁复过程。
应用:用CD11c微珠分选的DCs可用于表型和功能分析,如:在不同实验小鼠模型中研究T细胞活化、极化、诱导耐受。CD11c微珠可用于从脾脏和**结1、派尔结(Peyer’s结)2、结肠固有层3、骨髓4、上皮5、肺脏6、肝脏7、大脑8中分选高纯度DCs。
分选柱:阳性分选:MS、LS 、XS或autoMACSTM分选柱。去除分选:LD、CS、D或autoMACS分选柱。
CD11c+ DCs were isolated from mouse spleen cell suspension using CD11c MicroBeads, a MiniMACS™ Separator, and two MS Columns. The cells were fluorescently stained with CD11c-PE and Anti-MHC Class II-FITC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence. Unseparated fraction cells Isolated CD11c+ dendritic
CD11c+ DCs were isolated from mouse spleen cell suspension using CD11c MicroBeads, a MiniMACS™ Separator, and two MS Columns. The cells were fluorescently stained with CD11c-PE and Anti-MHC Class II-FITC. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Unseparated fraction cells Isolated CD11c+ dendritic