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  • 产品名称:美国伯乐BIO-RAD Immun-Star™ HRP 化学发光试剂盒 Clarity™ Western ECL Substrate, 200 ml

  • 产品型号:1705060
  • 产品厂商:伯乐/Bio-rad
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简单介绍:
美国伯乐BIO-RAD Immun-Star™ HRP 化学发光试剂盒 Clarity™ Western ECL Substrate, 200 ml 化学发光试剂盒 伯乐 BIO-RAD 1705060 美国伯乐BIO-RAD Immun-Star™ HRP 化学发光试剂盒 Clarity™ Western ECL Substrate, 200 ml现货 美国伯乐BIO-RAD Immun-Star™ HRP 化学发光试剂盒 Clarity™ Western ECL Substrate, 200 ml代理 美国伯乐BIO-RAD Immun-Star™ HRP 化学发光试剂盒 Clarity™ Western ECL Substrate, 200 ml优惠
详情介绍:

美国伯乐BIO-RAD Immun-Star? HRP 化学发光试剂盒 Clarity? Western ECL Substrate, 200 ml
化学发光试剂盒 伯乐 BIO-RAD
1705060

美国伯乐BIO-RAD Immun-Star? HRP 化学发光试剂盒 Clarity? Western ECL Substrate, 200 ml现货 美国伯乐BIO-RAD Immun-Star? HRP 化学发光试剂盒 Clarity? Western ECL Substrate, 200 ml代理 美国伯乐BIO-RAD Immun-Star? HRP 化学发光试剂盒 Clarity? Western ECL Substrate, 200 ml优惠

化学发光Western杂交检测,是同位素检测的一种高灵敏度的替代方法。酶标记抗体取代了放射性标记抗体,当它作用于底物时,可产生光信号。 Immun-Star? WesternC? 和 Immun-Star HRP 化学发光剂盒利用 HRP 共轭二抗使底物氧化发光以产生可使用胶片或成像仪捕捉的光信号。增强剂可加强信号输出并增加信号持续时间,在NC膜或PVDF膜上生成快速、灵敏的结构。
Immun-Star WesternC 化学发光试剂盒

Immun-Star WesternC 化学发光试剂盒可以通过CCD成像进行Western杂交免疫检测,它具有发光强度高,信号持续时间长的特点,能充分发挥CCD成像的优势。使用方便的试剂盒包括可以可与HRP反应发光的过氧化物缓冲液和luminol发光增强剂。 Immun-Star WesternC 化学发光试剂盒与任何 HRP偶联二抗相容,工作液至少可稳定 24 小时。

主要功能和优点包括:

  • 飞克级的高灵敏度
  • 工作液保持 24 小时稳定性,允许进行多次反应
  • 信号持续时间为 24 小时,以便通过多次曝光进行优化
  • 与 PVDF 和硝化纤维素膜兼容
  • 使用CCD图像仪如 ChemiDoc? XRS 和 VersaDoc? MP 系统成像可获得*优效果
  • 与 Precision Plus Protein? WesternC 标准品兼容

Immun-Star HRP 化学发光试剂盒

Immun-Star HRP 试剂盒包含HRP偶联二抗。 HRP催化的底物氧化反应产生光信号,可被胶片或者成像系统捕获。Luminol增强剂进一步加强信号输出,能检测到皮克级的靶蛋白并把信号持续时间延长到 6–8 小时。Immun-Star HRP 试剂盒是替代同位素检测的一种高灵敏度、快速的检测方法。主要特点包括:

  • 低皮克级的高灵敏度
  • 工作液在室温下保持 24 小时稳定性
  • 信号持续时间为 6–8 小时,曝光时间短(大约 30 秒)
  • 与 PVDF 和硝化纤维素膜兼容
  • 印迹膜可多次洗脱和再杂交
可提供两种类型的试剂盒,分别含有山羊抗小鼠和山羊抗兔偶联二抗。

化学发光

化学发光检测。 与目标蛋白结合的二抗上偶联一种酶,当酶与底物作用时会发出光信号。
Clarity Western ECL Substrate Performance

Comparison of chemiluminescent substrate sensitivity. A, five different western blots were loaded with the same protein loads of purified alcohol dehydrogenase (ADH). Blots were incubated with substrates per manufacturer recommendations, then imaged for 10 sec on the ChemiDoc? MP Imaging System. B, the signal-to-noise ratio for each band is depicted in bar graph form. Clarity and ECL Plus provided the best signal-to-noise ratios across all protein loads.
Clarity Max Western ECL Substrate Performance

Comparison of chemiluminescent substrate signal intensity. A, five different western blots were loaded with the same protein loads of purified IKB alpha. Blots were incubated with substrates per manufacturer recommendations, then imaged for 70 sec on the ChemiDoc Touch Imaging System.B, the signal-to-noise ratios for the bands in the boxed area for Clarity Max, ECL Select, and SuperSignal West Femto are depicted in bar graph form. Clarity Max and ECL Select provided the best signal-to-noise ratios across all protein loads. ND, not determined; signal at 30 pg for SuperSignal West Femto was not detectable above background noise.
技术指标:
Lower detection limit Clarity: Mid-femtogram level
Clarity Max: Low-femtogram level
Signal duration Clarity: 24 hr
Clarity Max: 2 hr
Primary detection method Digital or film imaging
Suggested antibody dilution Primary: 1:1,000–1:50,000;
Secondary: 1:50,000–1:250,000
Recommended membrane Nitrocellulose, PVDF, or LF PVDF
订购信息:

Clarity? Western ECL Substrate, 200 ml 
1705060 
Contains 100 ml Clarity Western Peroxide Reagent and 100 ml Clarity Western Luminol/Enhancer reagent
Clarity? Western ECL Substrate, 500 ml 
1705061 
Contains 250 ml Clarity Western Peroxide Reagent and 250 ml Clarity Western Luminol/Enhancer Reagent


   

Clarity Max? Western ECL Substrate, 100 ml 
1705062 
Pkg of 1, contains 50 ml Clarity Max Western Peroxide Reagent and 50 ml Clarity Max Luminol/Enhancer Reagent.
Immun-Blot® PVDF Membrane, Roll, 26 cm x 3.3 m 1620177 Pkg of 1 roll
Immun-Blot® PVDF Membrane, Roll, 26 cm x 3.3 m 
1620177 
Pkg of 1 roll, 0.2 μm, 26 cm x 3.3 m, bulk membrane for high binding (150–160 μg/cm2) immunoblotting.
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