- Cat. No.
- BTE 003-02
- Product name
- Anti-Procollagen type IIA N-terminal propeptide (human, PIIANP)
- Description
-
Mouse monoclonal antibody
- Specificity
BTE 003-02 is specific for human PIIANP
- Immunogen
Recombinant expressed PIIANP
- Application
- ELISA, IHC-P
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
IgG1/k
- Gene ID
- 1277
- Clone number
- NA
- Epitope
Epitope is located at the amino-terminal part of procollagen type IIA
- Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
Collagen type II is the most abundant protein of cartilage matrix. Two isoforms exist and collagen type IIA is involved in early development of cartilage in embryos. The collagen is produced from type II procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. Procollagen type II N-terminal propeptide (PIIANP) is considered a marker of cartilage synthesis and the normal concentration in serum is approximately 30ng/mL.
- Application 1
ELISA
BTE 003-02 also reacts with recombinant PIIANP coated directly onto the microtiter well- Application 2
- IHCBTE 003-02 diluted in 10%FCS/TBS is well suited for immunohistochemistry on formalin-fixed, paraffin-embedded fetal tissue.
Immunohistochemical staining of a formalin-fixed, paraffin-embedded fetal bone anlage in the hand (around week 10) with BTE 003-02. 
Immunohistochemical staining of a formalin-fixed, paraffin-embedded biopsy from a chondroblastoma, showing specific staining of tumor cells with AntibodyShop product BTE 003-02 developed with peroxidase-labeled goat anti-mouse Ig (blue color).
