- Cat. No.
- BTE 004-09
- Product name
- Anti-Pregnancy associated plasma protein A (human, PAPP-A)
- Description
-
Mouse monoclonal antibody
- Specificity
BTE 004-09 is specific for native human PAPP-A. Heat and SDS denatured PAPP-A is also recognized, but not acid treated PAPP-A.
- Immunogen
Purified human PAPP-A
- Application
- ELISA, WB, IHC-P, IHC-F
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
IgG2b/k
- Gene ID
- 5069
- Clone number
- NA
- Epitope
The epitop of BTE 004-09 is different from ABS 006-01 and ABS 006-24. The epitope is located on the PAPP-A subunit of the heterotetramer.
- Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
Pregnancy-associated plasma protein-A (PAPP-A, Insulin-like growth factor binding protein-4 protease, PAPP-A-proMBP complex) is a large heterotetrameric glycoprotein of approximately 500 kDa, which was first discovered in serum from pregnant women. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Low maternal serum levels of PAPP-A in first trimester biochemical screening is used as a marker of Down’s syndrome (trisomy 21).
- Application 1
ELISA
In sandwich ELISA the optimal combination is BTE 004-09 used as coating antibody and biotinylated ABS 006-24 used as detection antibody. BTE 004-09 can also be used as detection antibody in sandwich ELISA in combination with a polyclonal antibody towards PAPP-A in coat.- Application 2
WB
BTE 004-09 detect both reduced and unreduced PAPP-A in Western Blotting, though reaction to unreduced is less than that of ABS 006-01 and ABS 006-24.- Application 3
IHC
BTE 004-09 can be used in IHC.
Staining is restricted to the syncytiotrophoblast of the chorionic villi in frozen human placenta tissue, stained with BTE 004-09. 
Staining is restricted to the syncytiotrophoblast of the chorionic villi in paraffin embedded human placenta tissue, stained with BTE 004-09.
