- Cat. No.
- HYB 230-01B
- Product name
- Anti-Antithrombin III (human, native)
- Description
-
Mouse monoclonal antibody, Biotinylated
- Specificity
HYB 230-01 is specific for human Antithrombin III (AT III) native form
- Immunogen
Antithrombin III isolated from human plasma
- Application
- ELISA
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
IgG1/k
- Clone number
- 1 B7
- Epitope
Epitope specificity differs from that of HYB 230-04, HYB 228-10 and HYB 228-12.
- Content
Available in 100 µL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Biotinylated
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.14 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
Antithrombin III (AT III) is a single chain glycoprotein of molecular mass 58 kDa with four glycosylation sites and three intra-molecular disulfide bonds. AT III is normally present in human plasma at a concentration of approx. 12.5 mg/dl. It is the major inhibitor of thrombin. Inactivation of thrombin by AT-III occurs by formation of a covalent bond resulting in an inactive 1:1 stoichiometric complex between the two components.
- Application 1
ELISA
HYB 230-01 reacts strongly with native AT III. Strong reaction is seen in ELISA with native AT III coated directly onto the microtiter well. In a sandwich ELISA setup, HYB 230-01B acts as a detection antibody in conbination with ABS 014-22 as capture antibody to measure Thrombin-Antithrombin (TAT) complexes.
The calibration curve of a sandwich assay for TAT (Thrombin-antithrombin) using ABS 014-22 as capture antibody and HYB 230-01B as the biotinylated detection antibody.
