- Cat. No.
- ABS 039-32
- Product name
- Anti-NGAL (rat)
- Description
-
Mouse monoclonal antibody
- Specificity
ABS 039-32 binds rat NGAL (neutrophil gelatinase-associated lipocalin)
- Immunogen
Recombinant rat NGAL precursor
- Application
- ELISA, WB
- Species reactivity (positive)
- Rat
- Species reactivity (negative)
- Human, pig, dog, mouse
- Subclass
IgG1/k
- Gene ID
- 170496
- Clone number
- 32
- Epitope
Not determined
- Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
Rat NGAL, so called because it is the ortholog of human neutrophil gelatinase-associated lipocalin (NGAL), is also called lipocalin 2, 25-kDa alpha 2-microglobulin-related protein or neu-related lipocalin. It is expressed by neutrophils and various epithelial cells, including tubular cells of the kidney. It is involved in physiological processes such as the binding of siderophore iron and tissue growth and regeneration. It is up-regulated in a variety of pathological situations including infection, inflammation, renal injury and certain adenocarcinomas.
- Application 1
ELISA
ABS 039-32 binds free rat NGAL in solution. ABS 039-32 (as biotinylated detection antibody) forms a sandwich ELISA pair with ABS 039-15 (as capture antibody) for measuring rat NGAL,(measuring range 4-400 pg/mL in an optimized assay).
The figure illustrates the calibration curve of a sandwich assay with ABS 039-15 as the capture antibody and ABS 039-32B as the biotinylated detection antibody. - Application 2
WB
ABS 039-32 can be used in Western blotting.
1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A
