- Cat. No.
- ABS 062-14B
- Product name
- Anti-NGAL (human)
- Description
-
Mouse monoclonal antibody, biotinylated
- Specificity
ABS 062-14 is specific for human NGAL (neutrophil gelatinase-associated lipocalin)
- Immunogen
Human recombinant NGAL coupled to carrier
- Application
- ELISA
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
IgG2a/k
- Gene ID
- 3934
- Clone number
- 14
- Epitope
Differs from those of HYB 211-01, the ABS 038 series of antibodies and ABS 062-33.
- Content
50 µL, 1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Biotinylated
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, with 0.14 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. Expression of NGAL in the kidney is dramatically increased by acute kidney injury.
- Application 1
ELISA
When ABS 062-14 is used as biotinylated detection antibody with ABS 062-33 as capture antibody, the assay is specific for NGAL monomer, cross-reacting <1% with="" ngal="" homodimer.
The calibration curve of a sandwich assay for monomer human NGAL using ABS 062-33 as the capture antibody and ABS 062-14B as the biotinylated detection antibody. (1).
1. Bangert et al (2013) The Monomer is the Major Form of NGAL in Urine and Plasma in Severe Sepsis J Am Soc Nephrol 24: 368A
