For In Vitro Diagnostic Use.
The MicroVue 25-OH Vitamin D EIA is an immunocapture assay for the determination of total 25-OH Vitamin D in human serum.
Vitamin D is a group of fat-soluble secosteroids which are produced in the skin of vertebrates after exposure to ultraviolet light from the sun, available from artificial sources, or occur naturally in a small range of foods. Total 25-OH Vitamin D is a combined measurement of two major forms, vitamin D2 (ergocalciferol) and vitamin D3 (cholecalciferol); known collectively as calciferol. When synthesized in the kidneys, calciferol circulates as a hormone; regulating, among other things, the concentration of calcium and phosphate in the bloodstream, promoting the healthy mineralization, growth and remodeling of bone.
Features & Benefits
| Feature | Benefit |
|---|
Product Specifications
| Product Spec Name | Product Spec Data |
|---|---|
|
Format
|
ELISA |
|
Test/kit
|
96 wells/plate |
|
Sample type
|
Serum |
Performance Characteristics
Method: Immuno-capture
Analyte: 25-OH Vitamin D2 & D3
Specimen: Serum
Specimen Volume: 50 µL
Limit of Detection: 1.5 ng/mL
Dynamic Range: 0-133 ng/mL
Precision CVs within-run: 2.7%-5.7%
Precision CVs between-run: 4.3%-4.7%
Incubation Time: 3 Hours
About the Assay
Assay Steps
- Add 50 µL of Standard, Control or sample
- Add 150 µL of the Assay Buffer
- Place on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 2 hours
- Wash 3 times with 1X Wash Buffer
- Add 200 µL of working HRP conjugate
- Place on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 30 minutes
- Wash 3 times with 1X Wash Buffer
- Add 100 µL of the TMB Substrate
- With appropriate cover to avoid light exposure, place the microplate(s) on a plate shaker at 300-700 rpm at room temperature (18°C to 28°C) for 15 minutes
- Add 100 µL of Stop Solution and read at 450 nm
- Data Reduction: 4 parameter logistics, or B/B0% interpolation
Ordering Information
| Catalog Number | Description |
|---|---|
|
8046
|
96 Wells/Plate |
