For Research Use Only. Not for use in diagnostic procedures.
The MicroVue Creatinine assay is offered as a convenience for researchers with no other means to perform the test. Urine creatinine is required to normalize the MicroVue urine resorption assays, DPD, PYD, HP, urine Total Dpd.
Creatinine is a molecule with a molecular weight of 113.1 g/mol. Creatinine is found in muscle tissues and is excreted into the circulation at a constant rate. It is removed from the plasma by globular filtration and excreted into the urine. The MicroVue Creatinine assay is a quantitative, colorimetric assay based on a modified Jaffe method where alkaline picrate forms a colored solution in the presence of creatinine.
Features & Benefits
Feature | Benefit |
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Product Specifications
Product Spec Name | Product Spec Data |
---|---|
Format
|
Colorimetric assay based on Jaffe method |
Test/kit
|
96 wells/plate |
Sample type
|
Urine |
About the Assay
Format
96-well microplate with reagents sufficient to test 42 samples in duplicate
Species Reactivity
Creatinine values from non-human species have not been evaluated with the MicroVue Creatinine kit.
Assay Steps
Add 50 µL 1:40 diluted Standards, Controls and samples
Add 150 µL Working Color Solution
Incubate 30 minutes at 18°C to 28°C
Read Optical Density at 490 nm
Ordering Information
Catalog Number | Description | Kit Size / Case Size |
---|---|---|
8009
|
ELISA Kit |
96 Wells/Plate
|