Features & Benefits
| Feature | Benefit |
|---|
Product Specifications
| Product Spec Name | Product Spec Data |
|---|---|
|
Format
|
ELISA |
|
Test/kit
|
96 wells/plate |
|
Sample type
|
Serum |
Performance Characteristics
Method: Competitive
Analyte: Pyridinoline
Specimen: Serum
Specimen Volume: 25 µL post-filtration
LOD*: 0.4 nmol/L
LLOQ*: 0.75 nmol/L
HLOQ*: 12 nmol/L
Precision CVs within-run: 6-15%
Precision CVs between-run: 9-12%
Incubation Time: 20-26 Hours
Cross-reactivity: Free PYD 100% Free DPD 0% PYD/DPD peptides < 2.5% L-arginine 0.0004% (see Package Insert)
*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification
About the Assay
Format
96 well microtiter plate, with reagents sufficient to test 40 samples in duplicate
Antibody Specificity
Free pyridinoline cross-links, some cross-reactivity to glutamine and arginine (0.5 nmol/L signal)
Species Reactivity
Baboon, Cat, Cow, Dog, Guinea Pig, Horse, Human, Rhesus Macaque, Mouse, Rat
No reactivity detected with Rabbit
Assay Steps
Spin serum in Spinfilters (included) for 30 minutes
Add 50 µL Reagent 1 to plate
Add 25 µL serum, diluted Standards, diluted Controls
Add 75 µL cold Pyridinoline Antibody
Incubate overnight at 2°C to 8°C
Wash 3 times with 1x Wash Buffer
Add 150 µL Enzyme Conjugate
Incubate 1 hour at room temperature
Wash 3 times with 1x Wash Buffer
Add 150 µL working Substrate Solution
Incubate 40 +/– 5 minutes at room temperature
Add 100 µL Stop Solution
Read at 405 nm
Data Reduction: 4 Parameter
Ordering Information
| Catalog Number | Description |
|---|---|
|
8019
|
96 Wells/Plate |
