| Molecular Weight: | 237.26 |
| Formula: | C14H11N3O |
| Purity: | ≥ 98% |
| CAS#: | 300586-90-7 |
| Solubility: | DMSO up to 100 mM |
| Chemical Name: | N-(1H-pyrrolo[2,3-c]pyridin-5-yl)benzamide |
| Storage: | Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year. |
Biological Activity:
OAC1 is a potent and selective small molecule to activate both Oct4 and Nanog promoter-driven luciferase reporter genes, identified from a cell-based high-throughput screening. When added to the reprogramming media along with the four reprogramming factors (Oct4, Sox2, Klf4, and c-Myc), OAC1 can significantly enhance iPSC reprogramming efficiency (20-fold higher than that induced by the 4F alone) and accelerate the reprogramming process. The iPSC colonies derived using OAC1 along with the four factors exhibited typical ESC morphology, gene-expression pattern, and developmental potential. OAC1 seems to enhance reprogramming efficiency in a unique manner, independent of either inhibition of the p53-p21 pathway or activation of the Wnt-β-catenin signaling. OAC1 increases transcription of the Oct4-Nanog-Sox2 triad and Tet1, a gene known to be involved in DNA demethylation. OAC1 may be used for large-scale iPSC generation for various applications.
How to Use:
- In vitro: OAC1 was used at 1 µM final concentration in iPSC culture media for 7 days (2 days after viral transduction with the four reprogramming factors).
- In vivo: n/a
Reference:
- 1. Li W, et al. Identification of Oct4-activating compounds that enhance reprogramming efficiency. (2012) Proc Natl Acad Sci USA.109(51):20853-8.
Products are for research use only. Not for human use.
