产品详情
简单介绍：
Hh-Ag1.5,HedgehogAgonist
详情介绍：
Product Information
| Molecular Weight: | 526.04 |
| Formula: | C28H26ClF2N3OS |
| Purity: | ≥98% |
| CAS#: | 612542-14-0 |
| Solubility: | DMSO up to 50 mM |
| Chemical Name: | 3-chloro-4,7-difluoro-N-(4-(methylamino)cyclohexyl)-N-(3-(pyridin-4-yl)benzyl)benzo[b]thiophene-2-carboxamide |
| Storage: | Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year. |
Biological Activity:
Hh-Ag1.5 is one of the most potent and selective small-molecule agonists of the Hedgehog pathway, targeting Smoothened (Smo) with an EC50 ~1 nM. Studies showed that it has more potent activity than the recombinant Hh protein in activating Smo and hedgehog pathway.
How to Use:
- In vitro: Hh-Ag1.5 was used at 25 nM to induce Hh pathway activation in the reporter gene assay. It was suggested to use at 0.1 µM to replace recombinant Hh protein.
- In vivo: n/a
Reference:
- 1. Frank-Kamenetsky M, et al. Small-molecule modulators of Hedgehog signaling: identification and characterization of Smoothened agonists and antagonists. (2002) Journal of Biology (1): 10.2-10.19.
- 2. Borzillo GV, et al. The Hedgehog Signaling Pathway as a Target for Anticancer Drug Discovery. (2005) Current Topics in Medicinal Chemistry (5), 147-157.
- 3. Pan S, et al. Discovery of NVP-LDE225, a Potent and Selective Smoothened Antagonist. ACS Med. Chem. Lett., 2010, 1 (3), pp 130–134.
Products are for research use only. Not for human use. |
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