产品详情
简单介绍：
IWP-2,Porcupine(Wnt)Inhibitor
详情介绍：
Product Information
| Molecular Weight: | 466.60 |
| Formula: | C22H18N4O2S3 |
| Purity: | ≥98% |
| CAS#: | 686770-61-6 |
| Solubility: | DMSO up to 10 mM |
| Chemical Name: | N-(6-methylbenzo[d]thiazol-2-yl)-2-((4-oxo-3-phenyl-3,4,6,7-tetrahydrothieno[3,2-d]pyrimidin-2-yl)thio)acetamide |
| Storage: | Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year. |
Biological Activity:
IWP-2 is a potent and highly selective Wnt signaling antagonist with an IC50 ~ 27 nM in the L-Wnt-STF cellular assay. It prevents palmitoylation of Wnt proteins by Porcupine (a membrane-bound O-acyltransferase), thereby blocking Wnt protein secretion and activity. It blocks Wnt-dependent phosphorylation of Lrp6 receptor and Dvl2, and β-catenin accumulation. IWP-2 is a useful chemical probe in both regenerative medicine and anticancer efforts.
How to Use:
- In vitro: IWP-2 was used at 1-2 µM in vitro and in cellular assays.
- In vivo: n/a
Reference:
- 1. Chen B, et al. Small molecule-mediated disruption of Wnt-dependent signaling in tissue regeneration and cancer. (2009) Nat Chem Biol. 5(2):100-7.
- 2. ten Berge D, et al. Embryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells. (2011) Nat Cell Biol. 13(9):1070-5.
- 3. Lian X, et al. Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling. (2012) Proc Natl Acad Sci USA. 109(27):E1848-57.
Products are for research use only. Not for human use.
相关文章
