| CAS#: | 91396-88-2 |
| Purity: | ≥ 98% |
| Formula: | C12H11N3O |
| Molecular Weight: | 213.24 |
| Solubility: | DMSO up to 100 mM |
| Chemical Name: | N'-phenylisonicotinohydrazide |
| Storage: | Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year. |
Biological Activity:
PluriSIn#1 is a potent small molecule to selectively eliminate human pluripotent stem cells/hPSCs while sparing a large array of tissue-specific stem/progenitor and differentiated cells. It was identified by a high-throughput screening against undifferentiated human ESCs. PluriSIn#1 induces ER stress, protein synthesis attenuation, and apoptosis in hPSCs specifically. Mechanistic characterizations uncovered that PluriSIn#1 is a specific inhibitor of stearoyl-coA desaturase (SCD1), the key enzyme in oleic acid biosynthesis, revealing a unique role for lipid metabolism in hPSCs. PluriSIn#1 is also cytotoxic to mouse blastocysts, indicating that the dependence on oleate is inherent to the pluripotent cells. Application of PluriSIn#1 prevented teratoma formation from tumorigenic undifferentiated cells. It can serve as a very useful reagent to enhance the safety of hPSC-based treatments and facilitate safe transplantation of larger cell numbers.
How to Use:
In vitro: PluriSIn#1 was used at 10 µM final concentration in culture media for 4 days to eliminate ESCs or iPSCs.
In vivo: n/a
Reference:
1. Ben-David U, et al. Selective Elimination of Human Pluripotent Stem Cells by an Oleate Synthesis Inhibitor Discovered in a High-Throughput Screen. (2013) Cell Stem Cell. In press.
Products are for research use only. Not for human use.
