产品详情
简单介绍：
PS48---PDK1Activator
详情介绍：
Product Information
| Molecular Weight: | 286.75 |
| Formula: | C17H15ClO2 |
| Purity: | ≥98% |
| CAS#: | 1180678-32-7 |
| Solubility: | DMSO up to 100 mM |
| Chemical Name: | 5-(4-Chloro-phenyl)-3-phenyl-pent-2-enoic acid |
| Storage: | Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year. |
Biological Activity:
PS48 is a novel PDK1 (phosphoinositide-dependent protein kinase 1) activator (Kd = 10.3 μM), which binds to the HM/PIF binding pocket rather than the ATP-. PS48 has been used to enhance iPSC reprogramming from human epidermal keratinocytes and endothelial cells.
How to Use:
- In vitro: PS48 was used at 5 µM concentration in cell culture.
- In vivo: n/a
Reference:
- 1. Hindie V, et al. Structure and allosteric effects of low-molecular- weight activators on the protein kinase PDK1. (2009) Nature Chem Biol. 5: 758-764.
- 2. Stroba A, et al. 3,5-Diphenylpent-2-enoic acids as allosteric activators of the protein kinase PDK1: structure-activity relationships and thermodynamic characterization of binding as paradigms for PIF-binding pocket-targeting compounds. (2009) J Med Chem. 52(15):4683-93.
- 3. Zhu S, et al. Reprogramming of Human Primary Somatic Cells by Oct4 and Chemical Compounds. (2010) Cell Stem Cell. 7 (6)651-655.
Products are for research use only. Not for human use. |
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