产品详情
简单介绍：
TAME,UbiquitinLigaseAPC/CInhibitor
详情介绍：
Product Information
| Molecular Weight: | 378.87 |
| Formula: | C14H22N4O4S.HCl |
| Purity: | ≥98% |
| CAS#: | 1784-03-8 |
| Solubility: | DMSO and water up to 100 mM |
| Chemical Name: | (S)-methyl 5-guanidino-2-(4-methylphenylsulfonamido)pentanoate |
| Storage: | Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year. |
Biological Activity:
TAME is a competitive inhibitor of the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C). It promotes Cdc20 autoubiquitination and inhibits cyclin proteolysis in mitotic Xenopus oocyte extracts (IC50 ~ 12 μM) and blocks cyclin degradation in interphase extract.
How to Use:
In vitro: TAME was used at 50-200 µM final concentration in various in vitro assays.
In vivo: n/a
Reference:
- 1. Zeng X, et al. Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. (2010) Cancer Cell. 18(4):382-95.
- 2. Zeng X, et al. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. (2012) Nat Chem Biol. 8(4):383-92.
Products are for research use only. Not for human use.
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