| Clonality: Monoclonal | Source: Mouse | |||
| Detected band (by western) MW: 37 KD | Cross-Reactivity: Human | |||
| Concentration: 100 ug/100 ul | ||||
| Applications: ELISA 1/10000, WB 1/500 - 1/2000, IHC 1/200 - 1/1000, ICC 1/200 - 1/1000, FCM 1/200 - 1/400 | ||||
Alt. Names
H3 histone antibody, HIST1H3A antibody, Histone cluster 1, H3a antibody
Specificity:
BMI1 antibody detects endogenous levels of total BMI1
Immunogen:
Purified recombinant fragment of human BMI1 expressed in E. Coli
Purification:
Affinity-chromatography
Storage Condition and Buffer:
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt.
Polycomb complex protein BMI-1 is found a canonical self-renewal regulator. Tumor Formation and, more specifically, human colorectal cancer-initiating cells (CIC) are dependent on BMI-1. Down-regulation of BMI-1 inhibits the ability of colorectal CICs to self-renew, resulting in the abrogation of their tumorigenic potential. Targeting the BMI-1 self-renewal machinery represents a promising approach to the elimination of many cancer stem (and initiating) cells.
BIMI-1 antibody detects endogenous levels of total BMI-1, thus a good tool of BMI-1 related cancer study.
Please refer PDF file for Western, Immunofluorescence analysis,Chromatin immunoprecipitation analysis or other QC data.
Reference:
- Kreso A, et al. Self-renewal as a therapeutic target in human colorectal cancer. (2014) Nat Med. (1):29-36.
Product is for research use only. Not for human use.
