| Purity: | Affinity purification |
| Storage: | Store at -20 or -80°C. Avoid freeze/thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
Catalog No: A70026
Histone H4R3me2s Polyclonal Antibody
Application:
WB (1:500-1:2000), IF (1:50-1:200), IHC (1:50-1:200)
Cross-Reactivity: H, M, R, other (wide range)
Calculated MW: 11kDa
Species: Rabbit
Isotype: IgG
Purity: Affinity purification
Concentration: 1mg/mL
Storage: Store at -20 or -80°C. Avoid freeze/thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3
Immunogen Information
Immunogen:
A synthetic peptide corresponding to the amino terminus of histone H4 in which Arg3 is di-methylated
Gene ID: 8370
Swiss Prot: P62805
Synonyms: H3t; H3.4; H3/g; H3FT
Histone H4R3 monomethylation is catalyzed by PRMT1, its dimethylation is catalyzed by PRMT5, both symmetrically and asymmetrically (1, 2, 3). Histone H4R3 methylation/demethylation involves in a variety of biological events, such as gene transcription regulation, cellular response, embryonic development and cancer development (4, 5, 6). For different H4R3 methylation status, we have H4R3me1, asymmetric H4R3me2a, and symmetric H4R3me2s antibody available.
Reference:
1. Wang H, et al. Methylation of histone H4 at arginine 3 facilitating transcriptional activation by nuclear hormone receptor. (2001) Science 293(5531):853-7.
2. Stallcup MR, et al. Co-operation between protein-acetylating and protein-methylating co-activators in transcriptional activation. (2000) Biochem Soc Trans. 28(4):415-8.
3. Ancelin K, et al. Blimp1 associates with Prmt5 and directs histone arginine methylation in mouse germ cells. (2006) Nat Cell Biol. Jun; 8(6):623-30.
4. Li X, et al. H4R3 methylation facilitates β-globin transcription by regulating histone acetyltransferase binding and H3 acetylation (2010) Blood 11; 115(10):2028–2037.
5. Pal S, et al. Low levels of miR-92b/96 induce PRMT5 translation and H3R8/H4R3 methylation in mantle cell lymphoma. (2007) EMBO J. August 8; 26(15):3558–3569.
6. Yue M, et al. Histone H4R3 Methylation Catalyzed by SKB1/PRMT5 Is Required for Maintaining Shoot Apical Meristem. (2013) PLoS ONE 8(12): e83258.
