Complement convadase assay是一种用于确定生物材料补体激活的灵敏且特异性的方法。
在与血浆孵育过程中，补体因子与材料表面结合，然后形成补体合酶复合物。 洗涤后，将生物材料在含有特定显色底物的培养基中孵育，以去除未结合的补体蛋白。 通过测量 405 nm 的光密度来量化底物裂开。
◆ 应用
生物材料诱导的补体激活的测量
◆ 原理
将感兴趣的测试物质（理想情况下应知道表面积）作为血浆孵育。 在孵育过程中，补体因子与材料表面结合，然后形成补体自相酶复合物。
孵育后，洗涤样品并使用补体相容酶特异性显色底物分析补体相容酶活性。 显色速率取决于生物材料表面产生的相聚酶的量。
◆ 储存及稳定性
产品在 -20°C 下可稳定保存至少 6 周
◆ 参考文献
1. Fernández-Colino A, Wolf F, Rütten S, Schmitz-Rode T, Rodríguez-Cabello JC, Jockenhoevel S, Mela P. Small Caliber Compliant Vascular Grafts Based on Elastin-Like Recombinamers for in situ Tissue Engineering. Front Bioeng Biotechnol. 2019 Nov 19;7:340.
2. Wiegand C, Abel M, Hipler UC, Elsner P, Zieger M, Kurz J, Wendel HP, Stoppelkamp S. Hemostatic wound dressings: Predicting their effects by in vitro tests. J Biomater Appl. 2019 Apr; 33(9):1285-1297.
3. Naso F, Stefanelli U, Buratto E, Lazzari G, Perota A, Galli C, Gandaglia A. Alpha-Gal Inactivated Heart Valve Bioprostheses Exhibit an Anti-Calcification Propensity Similar to Knockout Tissues. Tissue Eng Part A. 2017 Oct; 23(19-20):1181-1195.
4. Aguiari P, Iop L, Favaretto F, Fidalgo CM, Naso F, Milan G, Vindigni V, Spina M, Bassetto F, Bagno A, Vettor R, Gerosa G. In vitro comparative assessment of decellularized bovine pericardial patches and commercial bioprosthetic heart valves. Biomed Mater. 2017 Feb 3; 12(1):015021.
5. Diaz-Gomez L, Alvarez-Lorenzo C, Concheiro A, Silva M, Dominguez F, Sheikh FA, Cantu T, Desai R, Garcia VL, Macossay J. Biodegradable electrospun nanofibers coated with platelet-rich plasma for cell adhesion and proliferation. Mater Sci Eng C Mater Biol Appl. 2014 Jul 1;40:180-8.
6. Gladitz M, Bauer J, Brückner P, Reinemann S, Wiegand C, Zieger M, Reddersen K, Hipler U, Frant M, Liefeith K, Radusch H. Antibacterial polyamides based on a dendritic zinc-hybrid with good biocompatibility showing reduced biofilm formation, BioNanoMaterials, 2014, 15(1-2), 31-46.
7. Jovanovic D, et al. Polyacylurethanes as novel degradable cell carrier materials for tissue engineering. Materials, 2011, 4.10: 1705-1727.
8. Jovanovic D, Engels GE, Plantinga JA, Bruinsma M, van Oeveren W, Schouten AJ, van Luyn MJ, Harmsen MC. Novel polyurethanes with interconnected porous structure induce in vivo tissue remodeling and accompanied vascularization. J Biomed Mater Res A. 2010 Oct; 95(1):198-208.
◆ 产品信息
|
产品名称 |
规格 |
货号 |
品牌 |
|
Complement Convertase Assay Test kit |
30 determinations |
K005 |
