- Description
- Data Sheet
A fully validated, highly specific affinity purified rabbit polyclonal antibody to Serine-2808 phosphorylated Ryanodine Receptor
The Ryanodine Receptor is a Ca2+ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca2+ to ryanodine receptor 2 (RyR2) opens the channel and Ca2+ stored in the SR moves through the channel into the cytosol to initiate muscle contraction. Abnormal structure and function of ryanodine receptors has been reported in failing hearts, with Ser-2808 phosphorylation appearing elevated in clinical situations which may contribute to the abnormal Ca2+ handling characteristics of cardiac muscle in these conditions. Ser-2808 can be phosphorylated in vitro by PKA or CaMKII, which is coincident with significant change in RyR2 channel function typified by an increased open probability, the abrogation of the inhibitory effects of CaM and Mg2+, dissociation of regulatory factors, expression of subconductance states and the expression of channel activity at diastolic Ca2+ concentrations. Serine-2808 in human RyR2 equates to Ser-2809 in rabbit RyR2.
Tested applications: Western blot (1:2000 dilution). Species recognition: human, rat, mouse, rabbit, canine and sheep.
| Description | Lyophilised Rabbit polyclonal IgG antibody specific to RyR2 phospho Ser-2808 (Ser-2809 in rabbit RyR2 sequence). |
| Immunogen | Synthetic peptide (YNRTRRIS(PO3H2)QT2810) conjugated to keyhole limpet haemocyanin. |
| Isotype | IgG |
| Purification | Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 100 μl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze – thaw cycles. |
| Species reactivity | Antibody recognises phosphorylated serine 2808 of the ryanodine receptor and binding is blocked in the presence of a peptide containing the Phosphor Ser-2808 epitope. Recognises Ser- 2808 phosphorylated RYR2 exclusively, and will not react with dephosphorylated RYR2. |
| Tested applications | WB |
| Recommended dilution | WB 1:2000 |
| Optimization | Optimal dilutions to be determined by end user |
| Storage | Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 100 μl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze – thaw cycles. |
| Storage | Lyophilised: 4 °C Reconstituted:-80 °C |
| Datasheet | A010-30AP Lot 0210-03
 A010-30AP Lot 0312-05 |
| MSDS | A010-30AP
|
| Regulatory statement | Product for research use only. Not intended for diagnostic or therapeutic use. |
