产品详情
简单介绍：
Ryanodine receptor 2 (RYR2) (pSer2814) pAb
详情介绍：
- Description
- Data Sheet
A fully validated, highly specific affinity purified rabbit polyclonal antibody to Serine-2814 phosphorylated Ryanodine Receptor
The Ryanodine Receptor (RyR2) is a Ca2+ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca2+ to ryanodine receptor 2 RyR2 opens the channel and Ca2+ stored in the SR moves through the channel into the cytosol to initiate muscle contraction. CaMKII, was able to phosphorylate Ser-2814 of RYR2 enhancing Ca2+- sensitivity and increasing open probability. Excessive phosphorylation of Ser-2814 leads to atrial fibrillation, arrhythmias and sudden death.
Tested applications: Western blot (1:1000 dilution). Species recognition: All mammalian species, when phosphorylated on Ser-2814 (or Ser-2815 rabbit).
| Description | Lyophilised rabbit polyclonal IgG antibody (A010-31AP) specific to RyR2 phospho ser-2814 |
| Immunogen | Synthetic peptide (TSQVS(PO3H2)VDAAH2819) corresponding to amino acids surrounding the phosphorylated serine residue at position 2814 of RyR2 (human) conjugated to KLH. |
| Isotype | IgG |
| Purification | Protein A affinity purified |
| Species reactivity | Dog, mouse, human: despite minor differences in sequence, this antibody interacts with antigen based on the human and mouse sequence. |
| Tested applications | WB. Not yet tested in other applications, therefore, optimal dilutions/concentrations should be determined by the user. Also effective in ELISA and IHC microscopy. |
| Recommended dilution | WB 1:1000 |
| Optimization | Optimal dilutions to be determined by end user |
| Storage | Lyophilised antibody is stable at 4°C when stored with desiccant. Reconstitute lyophilised powder in 100 µl of 18 MΩ H2O, aliquot and store frozen at -80°C for 1 year. Avoid freeze - thaw cycles. |
| Storage | Lyophilised: 4 °C Reconstituted:-80 °C |
| Datasheet | A010-31AP Lot 0611-03
 A010-31AP Lot 0314-01 |
| MSDS | A010-31AP
> |
| Regulatory statement | Product for research use only. Not intended for diagnostic or therapeutic use. |
相关文章
