产品详情
简单介绍：
Ryanodine receptor 2 (RYR2) (pSer2030) pAb
详情介绍：
- Description
- Data Sheet
A fully validated, highly specific rabbit polyclonal anti-serum to Serine-2030 phosphorylated Ryanodine Receptor
The ryanodine receptor (RyR2) is a Ca2+ channel of cardiac muscle that plays a central role in EC coupling. The binding of Ca2+ to RyR2 opens the channel and Ca2+ stored in the SR moves through the channel into the cytosol to initiate muscle contraction. PKA, and not CaMKII, was able to phosphorylate Ser-2030 of RYR2 in vitro and in isolated cardiac myocyte cells.
Tested applications: Western blot (1:5000 dilution). Species recognition: human, rat, mouse, rabbit, canine and sheep.
| Description | Lyophilised Rabbit polyclonal anti-serum containing IgG antibody specific for Ser-2030 (PKA) phosphorylated RYR2. |
| Immunogen | Synthetic peptide (TIRGRLLS(PO3H2)LVE2033) corresponding to amino acids surrounding the phosphorylated serine residue at position 2030 of RYR2 (human), conjugated to KLH. |
| Isotype | IgG |
| Purification | Raw serum |
| Species reactivity | The antibody recognises RYR2 phosphorylated at serine 2030 and binding is blocked in the presence of a peptide containing the phospho-Ser2030 epitope. |
| Tested applications | WB |
| Recommended dilution | WB 1:1000 |
| Optimization | Optimal dilutions to be determined by end user |
| Storage | Lyophilised antibody is stable at 4 °C when stored with desiccant. Reconstitute lyophilised powder in 50 µl of 18 MΩ H2O, aliquot and store frozen at -80 °C for 1 year. Avoid freeze - thaw cycles. |
| Storage | Lyophilised: 4 °C. Reconstituted:-80 °C |
| Datasheet | A010-32
 |
| MSDS | A010-32
|
| Regulatory statement | Product for research use only. Not intended for diagnostic or therapeutic use. |
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