- Description
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The Badrilla CAPTUREomeTM S-Palmitoylated Protein Kit is a simple and easy way of determining whether your protein of interest is post translationally modified with a palmitate group. The assay is based on the acyl-RAC (resin assisted capture) method and facilitates, with four simple steps – Block, Cleave, Capture and Analysis - determination of protein palmitoylation.
Kit highlights
- Enables capture of S-palmitoylated proteins.
- Kit designed to process 4 x 5 biological samples with paired negative controls (i.e. 40 samples in total).
- Convenient stopping point after blocking treatment.
- Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.
References
- Forrester M.T., et al. (2011) Site-specific analysis of protein S-acylation by resin assisted capture. J. Lipid Res. 52: 393-398
- Howie, J. et al (2014) Substrate recognition by the cell surface palmitoyl transferase DHHC5. PNAS 111: 17534–17539
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| Storage | Store at 4C. |
| Datasheet | K010-311-Protocol K010-311-MSDS |
| Regulatory statement | Product for research use only. Not intended for diagnostic or therapeutic use. |
Acyl PEG Exchange – An Important Advance in the Palmitoylation Toolbox
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There have been many recent advances in methodologies to detect the only reversible lipidation reaction of proteins, S-palmitoylation. This fatty acyl post translational modification has gained great interest in the research community as it is increasingly recognised as important in both health and disease. In this article I will briefly examine the development of the acyl exchange methodologies used by researchers to measure S-palmitoylation. I will focus in particular on the recently published Acyl PEG Exchange (APE) /Acyl PEGyl Gel Shift (APEGS) assay, paying particular attention to the advantages it offers scientists interested in exploring palmitoylation.
Coming Soon
For answers to common questions on palmitoylation check out our FAQ page
