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The new CAPTUREomeTM S-Palmitoylation Mini Kit from Badrilla represents an affordable gateway into the emerging field of palmitoylation research. The Mini Kit, based on acyl RAC (resin assisted capture) technology, enables rapid determination of protein palmitoylation status from as little as 1mg of total cell lysate protein.
Kit highlights include
- Competitively priced entry level kit.
- Enables quick and easy capture of S-palmitoylated proteins from as little as 1mg of total cell lysate protein.
- Kit designed to process 2 x 3 biological samples with paired negative controls (i.e. 12 samples in total).
- Convenient stopping point after blocking treatment.
- Assay suitable for downstream SDS-PAGE, western blot or mass spectrometry analysis.
References
- Forrester M.T., et al. (2011) Site-specific analysis of protein S-acylation by resin assisted capture. J. Lipid Res. 52: 393-398
- Howie, J. et al (2014) Substrate recognition by the cell surface palmitoyl transferase DHHC5. PNAS 111: 17534–17539
| Storage | Store at 4-8 0C. |
| Datasheet | K010-310-Protocol K010-310-MSDS |
| Regulatory statement | No |
Acyl PEG Exchange – An Important Advance in the Palmitoylation Toolbox
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There have been many recent advances in methodologies to detect the only reversible lipidation reaction of proteins, S-palmitoylation. This fatty acyl post translational modification has gained great interest in the research community as it is increasingly recognised as important in both health and disease. In this article I will briefly examine the development of the acyl exchange methodologies used by researchers to measure S-palmitoylation. I will focus in particular on the recently published Acyl PEG Exchange (APE) /Acyl PEGyl Gel Shift (APEGS) assay, paying particular attention to the advantages it offers scientists interested in exploring palmitoylation.
Coming Soon
For answers to common questions on palmitoylation check out our FAQ page
