产品详情
简单介绍：
Anti-Complement component C9 (human)
详情介绍：
- Cat. No.
- ABS 004-53
- Product name
- Anti-Complement component C9 (human)
- Description
-
Mouse monoclonal antibody
- Specificity
ABS 004-53 is specific for complement factor C9
- Immunogen
Complement factor C9
- Application
- ELISA
- Species reactivity (positive)
- Human
- Species reactivity (negative)
- Not determined
- Subclass
IgG1/k
- Gene ID
- 735
- Clone number
- C9 53
- Epitope
Not determined
- Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
Complement factor C9 is a 71kDa single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and has an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). The primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60 µg/mL, and the level is up regulated from hepatocytes by IFN-g.
- Application 1
ELISA
ABS 004-53 recognizes C9 in human serum diluted 1/50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 µg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate.
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