For Research Use Only. Not for use in diagnostic procedures.
The MicroVue Helical Peptide is a test to measure type I collagen helical peptide α1(I) 620-633 in urine, a marker for researching collagen degradation.
Type I collagen is degraded during bone resorption, releasing into circulation peptides of various molecular weights that are further degraded and/or released into urine. A peptide consisting of residues 620-633 derived from the helical region of the α1 chain was isolated from the urine of a patient with Paget's disease.
Features & Benefits
| Feature | Benefit |
|---|
Product Specifications
| Product Spec Name | Product Spec Data |
|---|---|
|
Format
|
ELISA |
|
Test/kit
|
96 wells/plate |
|
Sample type
|
Urine |
Performance Characteristics
Method: Competitive
Analyte: Helical Peptide α1(I) 620-633
Specimen: Urine
Specimen Volume: 20 µL
LOD*: 8 µg/L
LLOQ*: 20.2 µg/L
HLOQ*: 2514 µg/L
Precision CVs within-run: 4%-8%
Precision CVs between-run: 7%-17%
Incubation Time: 19-25 Hours
Cross-reactivity: Helical peptide α1(I) 620-633 100% Intact collagen I, II, III, IV < 1% Homologous peptides (see Package Insert)
*LOD = Limit of Detection, LLOQ = Lower Limit of Quantification, HLOQ = Higher Limit of Quantification
About the Assay
Format
96-well microplate with reagents sufficient to test 40 samples in duplicate
Antibody Specificity
Helical peptide α1(I) 620-633; no reactivity with intact collagen types I, II, III, IV or mixtures of amino acids; 90% reactivity with α1(III) homologous peptide; no reactivity with α1(I) or α2(II) homologous peptides
Species Reactivity
Rat, Mouse, Rabbit, Guinea Pig, Cat, Dog, Pig, Goat, Sheep, Cow, Horse, Rhesus Macaque, Cynomolgus Monkey, Baboon, Chimpanzee, Human Cell Culture
Assay Steps
Add 20 µL Standards, Controls, and samples
Add 150 µL Enzyme Conjugate
- Incubate 18-24 hours at 2°C to 8°C
- Wash 3 times with 1x Wash Buffer
- Add 150 µL Substrate
- Incubate 1 hour at room temperature
- Add 100 µL Stop Solution
- Read absorbance at 405 nm
- Data Reduction: 4 Parameter
- Correct for creatinine concentration
Ordering Information
| Catalog Number | Description | Kit Size / Case Size |
|---|---|---|
|
8022
|
ELISA Kit |
96 Wells/Plate
|
