Fluorescein DPc10 Inactive Peptide

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Product code: A010-517
Unit Size: 200µg net peptide

5Flu-GFCPDHKAAMVLFLDSVYGIEVQDFLLHLLEVGFLP

Negative control for fluorescein DPc10 peptide.

DPc10 inactive peptide is a 36-residue peptide that differs from DPc10 (Gly2460-Pro2495, rabbit) at one position (R2475S) and represents the sequence of a known CPVT mutant RYR2 domain. This peptide binds weakly or fails to bind to the DPc10 interface and does NOT affect channel function.

DPc10 inactive in the current form is not cell permeable. The product is useful in applications that permit access to RYR2 directly (via whole cell patch, skinned cells, black lipid membrane, vesicles, or upon delivery to cytoplasm).

Read DPc10 Publications

Peptide name	Fluorescein DPc10 Inactive Peptide
Peptide sequence	5Flu-GFCPDHKAAMVLFLDSVYGIEVQDFLLHLLEVGFLP
N-terminus	5-Carboxyfluorescein
C-terminus	Acid
Counter ions	Trifluroacetate (TFA)
Molecular weight	4093.046
HPLC analysis	Analysis carried out using a 100Å 4.6 x 150mm column, gradient from 10%-90% acetonitrile, in 15 minutes. 60 degC. Purity determined as 97%
MS analysis	Molecular ion predicted: 4393.046 . Observed: 4392.6 [M+3H]
Appearance	Yellow powdered solid
Solubilisation	DMSO
Storage	Store as supplied at -20°C for up to 1 year. Store desiccated. Make fresh solutions prior to use.
Datasheet	A010-517 Read DPc10 publications
MSDS	A010-517
Notes	The product was purified in acetonitrile and water containing 0.1% trifluoroacetic acid (TFA) prior to lyophilisation. The product will therefore be present as its trifluoroacetic acid salt.
Regulatory statement	Product for research use only. Not intended for diagnostic or therapeutic use.