脱氧核糖核酸酶 I 来源于牛胰腺
Deoxyribonuclease I from bovine pancreas
别名: DNase I, Deoxyribonuclease, 脱氧核糖核酸 5′-寡核苷酸-水解酶
lyophilized powder, Protein ≥85 %, ≥400 Kunitz units/mg protein
CAS号 9003-98-9
orm ：lyophilized powder
mol wt ：~31 kDa
composition：Protein, ≥85%
solubility ：0.15 M NaCl: soluble 5.0 mg/mL, hazy
foreign activity ：RNase ≤0.02%
适用：
• DNAse I is used to nick DNA as a first step to incorporate labeled bases into DNA. • DNAse can be used for Degradation of DNA templates in transcription reactions • DNAse has been used during the isolation of plasma membrane vesicles from Neurospora crassa cell culture. • Deoxyribonuclease I has also been used along with trypsin for the preparation of single cell suspension from rat testes. • Deoxyribonuclease I from bovine pancreas has been used in a study to compare several procedures for reducing RNase contamination in preparations of DNase. • Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effect of the composition of sodium dodecyl sulfate preparations on the renaturation of enzymes after polyacrylamide gel electrophoresis. • This DNase can be used for the removal of DNA from protein samples. • Preventing the loss of cells from undesired clumping during tissue dissociation: parts of the cells which are lysed, resulting in a release of DNA. Monomolecular DNA may cause clumping of cells. Addition of DNase I to the dissociation buffer leads to a degradation of this extracellular DNA, thereby.
用于从蛋白质样品中除去 DNA。
粤公网安备 44011202000535号
